3d Ultrasound Imaging
3d Ultrasound Imaging - Related Videos
08:52
3D Ultrasound Imaging: Fast and Cost-effective Morphometry of Musculoskeletal Tissue
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Cited by 34 •
2017
3D ultrasound imaging (3DUS) allows fast and cost-effective morphometry of musculoskeletal tissues. We present a protocol to measure muscle volume and fascicle length using 3DUS.
10:53
A Novel Application of Musculoskeletal Ultrasound Imaging
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Cited by 14 •
2013
We describe a new ultrasound-based vector tissue Doppler imaging technique to measure muscle contraction velocity, strain and strain rate with sub-millisecond temporal resolution during dynamic activities. This approach provides complementary measurements of dynamic muscle function and could lead to a better understanding of mechanisms underlying musculoskeletal disorders.
10:02
High-frequency Ultrasound Imaging of Mouse Cervical Lymph Nodes
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Cited by 6 •
2015
This protocol describes the application of high-frequency ultrasound (HFUS) for imaging mouse cervical lymph nodes. This technique optimizes visualization and quantification of cervical lymph node morphology, volume and blood flow. Image-guided biopsy of cervical lymph nodes and processing of lymph tissue for histological evaluation is also demonstrated.
09:00
Murine Echocardiography and Ultrasound Imaging
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Cited by 61 •
2010
This video demonstrates use of a rail-mounted high-frequency ultrasound probe to perform echocardiography on an anesthetized mouse. The methods describe both conventional two-dimensional and M-mode measurements of cardiac function in addition to newer, more powerful tools such as color Doppler, strain analysis, as well as general and targeted contrast imaging.
14:20
High-frequency Ultrasound Imaging of the Abdominal Aorta
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2023
Source: Amelia R. Adelsperger, Evan H. Phillips, and Craig J. Goergen, Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana High-frequency ultrasound systems are used to acquire high resolution images. Here, the use of a state-of-the-art system will be demonstrated to image the morphology and hemodynamics of small pulsatile arteries and veins found in mice and rats. Ultrasound is a relatively inexpensive, portable, and versatile method for the noninvasive...
FAQs
Acoustic impedance differences determine how much of an ultrasound pulse is reflected at tissue interfaces. The returning echo's timing indicates where a reflecting structure lies, while its strength provides information about the contrast between tissues. Combining these measurements across the acquired dataset allows researchers to distinguish boundaries and assess the resulting anatomy in three dimensions.
Probe motion and electronic beam steering provide different ways to sample anatomy from multiple positions or directions. Probe motion physically changes the measurement location, whereas electronic steering redirects beams without relying on the same type of manual movement. The resulting measurements supply spatial information needed to reconstruct volume and examine relationships among neighboring structures.
A volumetric view preserves relationships among structures that may be separated across individual 2D slices. This makes it possible to evaluate shape, estimate volume, and examine how an embryo, organ, vessel, or tissue region is arranged in space. The added spatial context can support clearer characterization of anatomy than reviewing isolated slices alone.
A basic workflow begins by transmitting high-frequency pulses into the specimen and recording the echoes that return from internal tissues. Measurements are gathered as the probe moves or as beams are electronically steered. The collected echo timing and strength data are then combined computationally to reconstruct a three-dimensional representation for visualization and measurement.
In biology, researchers can apply the method to embryos, organs, blood vessels, and organized tissue regions. The reconstructed volume supports measurements of shape, volume, and spatial relationships rather than only a single sectional view. These capabilities make the technique useful for developmental studies, investigations of disease-related anatomy, and analysis of tissue organization in living specimens.
Its noninvasive character and lack of ionizing radiation make 3D ultrasound imaging suitable for repeated observations of living specimens. Researchers can follow developmental changes or compare anatomy over time while preserving the ability to assess shape, volume, and spatial arrangement. This longitudinal use is especially relevant when biological processes need monitoring rather than a single endpoint measurement.