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High-frequency Ultrasound Imaging of Mouse Cervical Lymph Nodes
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Medicine
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JoVE Journal Medicine
High-frequency Ultrasound Imaging of Mouse Cervical Lymph Nodes

High-frequency Ultrasound Imaging of Mouse Cervical Lymph Nodes

DOI:
10.3791/52718-v

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10:02 min

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July 25, 2015

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, ,
1Department of Neurobiology and Anatomy,West Virginia University, 2Animal Models and Imaging Facility,West Virginia University, 3Mary Babb Randolph Cancer Center,West Virginia University

Chapters

  • 00:05Title
  • 01:42Preparations
  • 02:41Imaging the Mouse Cervical Lymph Nodes Using HFUS in B-Mode
  • 04:41Cervical Lymph Node Biopsy
  • 06:51Image Analysis of Cervical Lymph Nodes
  • 08:20Representative Results
  • 09:25Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the application of high-frequency ultrasound (HFUS) for imaging mouse cervical lymph nodes. This technique optimizes visualization and quantification of cervical lymph node morphology, volume and blood flow. Image-guided biopsy of cervical lymph nodes and processing of lymph tissue for histological evaluation is also demonstrated.

Tags

Keywords: High-frequency UltrasoundHFUSMouseCervical Lymph NodesB-mode ImagingPower DopplerFine Needle BiopsyLymph Node VolumeLymph Node Morphology

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