This protocol describes the application of high-frequency ultrasound (HFUS) for imaging mouse cervical lymph nodes. This technique optimizes visualization and quantification of cervical lymph node morphology, volume and blood flow. Image-guided biopsy of cervical lymph nodes and processing of lymph tissue for histological evaluation is also demonstrated.
Walk, E. L., McLaughlin, S. L., Weed, S. A. High-frequency Ultrasound Imaging of Mouse Cervical Lymph Nodes. J. Vis. Exp. (101), e52718, doi:10.3791/52718 (2015).