Antigen Tagged Lesion
Antigen Tagged Lesion - Related Videos
08:24
Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion
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Cited by 2 •
2021
While replication fork collisions with DNA adducts can induce double strand breaks, less is known about the interaction between replisomes and blocking lesions. We have employed the proximity ligation assay to visualize these encounters and to characterize the consequences for replisome composition.
06:57
Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells
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Cited by 9 •
2016
In this antigen-driven colitis model, OT-II CD4+ T cells expressing a red fluorescent protein were adoptively transferred into RAG-/- mice that express a green fluorescent protein in mononuclear phagocytes (MPs). The hosts were challenged with Escherichia coli (E.coli) expressing the ovalbumin protein (OVA) fused to a cyan fluorescent protein (CFP).
05:04
Viral Antigen Microarray Assay to Detect Antibody Isotypes in Serum Against Viral Antigen
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2025
In this video, we demonstrate the process for detecting antibody isotypes in multiple human serum samples using a microarray slide printed with antigenic subtypes of influenza virus strains.
09:22
Sectioning Mammary Gland Whole Mounts for Lesion Identification
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Cited by 13 •
2017
We developed a method to successfully remove, process, section, and stain, for histopathological evaluation, mammary tissue that had originally been fixed on slides as whole mounts. This method may promote the collection and evaluation of mammary gland whole mounts in reproductive and developmental test guideline studies.
08:16
Comparative Lesions Analysis Through a Targeted Sequencing Approach
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2019
This article describes a method to identify clonal and subclonal alterations among different specimens from a given patient. Although the experiments described here focus on a specific tumor type, the approach is broadly applicable to other solid tumors.
FAQs
Antibodies recognize and bind a selected antigen in preserved tissue, creating a molecular signal within or around the abnormal region. Researchers can then compare the detected antigen with the lesion’s visible cellular or structural changes. This linkage helps determine whether similar-looking abnormalities represent different lesion types or reflect different biological processes.
Labels make antibody-antigen complexes detectable after binding has occurred. Fluorescent labels provide one form of signal, whereas enzymatic labels provide another, allowing the same underlying recognition event to be visualized through different approaches. The choice of detectable label supports tissue localization and helps relate antigen distribution to the affected region.
An antigen’s position supplies information beyond its mere presence. Detection within the lesion can associate a molecular feature with affected cells or tissue, while detection around it may indicate a relationship at the lesion boundary or surrounding region. This spatial information helps researchers interpret pathology and connect cellular changes with underlying disease or infection-related processes.
Visible tissue abnormalities may not reveal which molecular feature produced them. Detecting a specific antigen adds a molecular criterion that can be compared across lesions. When one lesion contains a target antigen and another does not, researchers can use that difference to support lesion classification, investigate disease-associated proteins, or identify localized infectious agents.
A typical workflow begins with preserving the tissue, applying an antibody directed against the antigen of interest, and allowing detectable antibody-antigen complexes to form. Researchers then visualize the complexes using fluorescent, enzymatic, or another detectable label. Finally, they interpret the signal alongside the tissue abnormality to assess antigen location and biological significance.
These methods are useful when researchers need to connect tissue pathology with a defined antigen. They can support studies of disease-associated proteins, infectious agents, tumors, or immune reactions. By revealing where the target occurs in preserved tissue, the methods add molecular context to structural observations and help examine how lesions or related processes change over time.
The analysis can show whether a selected antigen is present, where it is located relative to the lesion, and how that distribution relates to cellular changes. It can therefore support lesion classification, localization of infectious agents, and investigation of disease-associated proteins. These outcomes help researchers interpret tissue pathology in experimental models and other biological studies.
By detecting relevant antigens in affected tissue, researchers can associate changing lesion features with molecular events during disease development. In infection studies, the approach can localize infectious agents; in tumor or immune-reaction studies, it can identify disease-associated proteins or related tissue signals. Repeated observations can help connect evolving pathology with underlying biological processes.