Cd54 Cd90 Enrichment
Cd54 Cd90 Enrichment - Related Videos
01:34
Environmental Enrichment for Rodents
0 Views •
2026
All animal procedures described here must be conducted in accordance with institutional animal ethics guidelines and approved by IACUC. All procedures must follow the principles of the 3Rs—Replacement, Reduction, and Refinement—and must be performed by trained personnel. Environmental enrichment involves objects and practices that encourage natural behaviors, improve psychological well-being, and promote better welfare in laboratory rodents. Environmental enrichment should be tailored to the...
01:27
Environmental Enrichment in Rabbits and Pigs
0 Views •
2026
All animal procedures described here must be conducted in accordance with institutional animal ethics guidelines and approved by IACUC. All procedures must follow the principles of the 3Rs—Replacement, Reduction, and Refinement—and must be performed by trained personnel. Importance of Environmental Enrichment Environmental enrichment is a fundamental component of laboratory animal care and welfare. It provides animals with opportunities to express species-specific behaviors, reduces stress, and...
09:30
A Protocol for Housing Mice in an Enriched Environment
0 Views •
Cited by 70 •
2015
Environmental enrichment for mice requires a complex and challenging setup, as well as comprehensive husbandry and handling techniques to assure robust metabolic and anti-cancer effects in the mice. This protocol provides detailed procedures to reproduce the above mentioned effects in mice.
02:03
Enrichment of Anode-Respiring Bacteria Using an On-Site Electrochemical System
0 Views •
2025
Source: Okamoto, A., et al. Self-standing Electrochemical Set-up to Enrich Anode-respiring Bacteria On-site. J. Vis. Exp. (2018)This video demonstrates the use of an on-site electrochemical system to enrich anode-respiring bacteria (ARB) from natural reservoirs. A redox gradient is established by placing the anode in oxygen-free deep water and the cathode in oxygen-rich surface water. ARB form biofilms and transfer electrons to the anode, which flows to the cathode, where oxygen is reduced,...
03:15
Separation and Enrichment of Extracellular Vesicles in the Presence of Virions
0 Views •
2026
Source: DeMarino, C., et al. Purification of High Yield Extracellular Vesicle Preparations Away from Virus. J. Vis. Exp. (2019)The video demonstrates the separation of EVs from virions and other contaminants by density gradient centrifugation, followed by capture and concentration with hydrogel nanoparticles. Washed fractions are then ready for downstream identification of those enriched in EVs.
FAQs
The paired-marker strategy enriches cells carrying selected surface features, making relationships between marker expression and cellular behavior easier to examine. Rather than analyzing the starting mixture as a single population, researchers can focus on a fraction with increased representation of CD54- and CD90-expressing cells. This supports studies of heterogeneity, phenotype, signaling, and behavior.
CD54, also called ICAM-1, connects the enriched population to questions about cell adhesion and signaling, while CD90, or Thy-1, helps identify cells of interest in studies of candidate stromal or stem-like populations. Their expression therefore serves both as a basis for separation and as a biological feature to relate to phenotype and tissue-specific behavior.
Both approaches use antibodies directed against the selected membrane proteins, but they separate labeled cells through different mechanisms. Magnetic-activated cell sorting uses magnetic separation, whereas fluorescence-activated cell sorting uses fluorescence-based separation. The choice provides alternative ways to obtain a population enriched for the desired marker profile before characterization, culture, or functional investigation.
A typical workflow begins with a heterogeneous biological sample and labels the relevant membrane proteins using specific antibodies. The labeled material then undergoes magnetic-activated or fluorescence-activated cell sorting, after which the selected fraction can be examined for marker expression and cellular properties. Researchers may subsequently use it for culture, characterization, or functional studies.
Post-enrichment analysis should determine whether the selected fraction shows the intended increase in CD54 and CD90 expression and how that profile relates to phenotype or behavior. Researchers can also examine whether the population provides useful evidence about cellular heterogeneity, adhesion, signaling, or tissue-specific biology. These comparisons connect the separation outcome with the study’s biological question.
This strategy is useful when a study needs to examine a defined marker-enriched fraction rather than the entire heterogeneous sample. Applications include investigating cell adhesion and signaling, characterizing cellular heterogeneity, and isolating candidate stromal or stem-like populations. The resulting cells can support downstream culture and functional studies that test how marker expression corresponds to cellular properties.