Zeocin Resistance Gene
Zeocin Resistance Gene - Related Videos
03:14
Assessing the Role of Gene Copy Number in the Evolution of Bacterial Antibiotic Resistance
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2026
Source: Escudero, J. A., et.al. Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance. J. Vis. Exp. (2018)This video demonstrates how escalating cefazidime exposure is used to compare the survival of three E. coli strains with different copies of the ampicillin resistance gene. It shows that only the strain with a multicopy plasmid survives at higher drug levels, highlighting how increased gene copy number accelerates resistance evolution.
08:17
VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance
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Cited by 22 •
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Virus-induced gene silencing is an useful tool for identifying genes involved in nonhost resistance of plants. We demonstrate the use of bacterial pathogens expressing GFPuv in identifying gene silenced plants susceptible to nonhost pathogens. This approach is easy, fast and facilitates large scale screening and similar protocol can be applied to studying various other plant-microbe interactions.
15:40
Forward Genetic Approach to Uncover Stress Resistance Genes in Mice — A High-throughput Screen in ES Cells
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2015
Stress resistance is one of the hallmarks for longevity and is known to be genetically governed. Here, we developed an unbiased high-throughput method to screen for mutations that confer stress resistance in ES cells with which to develop mouse models for longevity studies.
11:37
QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii
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Cited by 5 •
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Details are presented on how QTL mapping with a whole genome sequence based genetic map can be used to identify a drug resistance gene in Toxoplasma gondii and how this can be verified with the CRISPR/Cas9 system that efficiently edits a genomic target, in this case the drug resistance gene.
15:03
Building a Better Mosquito: Identifying the Genes Enabling Malaria and Dengue Fever Resistance in A. gambiae and A. aegypti Mosquitoes
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Cited by 2 •
2007
In this interview, George Dimopoulos focuses on the physiological mechanisms used by mosquitoes to combat Plasmodium falciparum and dengue virus infections. Explanation is given for how key refractory genes, those genes conferring resistance to vector pathogens, are identified in the mosquito and how this knowledge can be used to generate transgenic mosquitoes that are unable to carry the malaria parasite or dengue virus.
FAQs
The Ble protein protects cells by binding Zeocin before the antibiotic can interact with cellular DNA. This prevents the DNA strand breaks associated with Zeocin exposure, allowing cells that express the resistance protein to remain viable. The mechanism links survival directly to production of the marker encoded by the introduced genetic construct.
Zeocin exposure creates a survival difference between cells that carry the resistance gene and those that do not. Cells expressing the Ble protein can withstand the antibiotic, whereas susceptible cells are unable to continue growing under selection. As a result, the culture becomes enriched for cells that retained the introduced plasmid or expression vector.
Its usefulness comes from applying the same selectable-marker principle to multiple host organisms. The source material identifies bacteria, yeast, and mammalian cells as systems in which Zeocin resistance supports selection. This cross-organism applicability allows researchers to use the marker when developing genetically modified cultures or producing recombinant proteins in different biological contexts.
Zeocin belongs to the bleomycin family and can generate damaging DNA strand breaks when it interacts with DNA. Resistance does not remove the antibiotic from the experiment; instead, the Ble protein neutralizes its DNA-directed activity by binding Zeocin. This distinction explains why the marker protects selected cells while Zeocin remains useful as a selection pressure.
Researchers first place the resistance gene in a plasmid or expression vector and introduce that construct into the target cells. They then expose the resulting culture to Zeocin. Cells carrying and expressing the construct survive or continue growing, while susceptible cells are removed from the growing population, providing a selection-based indication of successful genetic modification.
The plasmid or expression vector serves as the genetic vehicle that carries the Zeocin resistance gene into the target cells. When the construct is introduced, the marker becomes associated with the engineered genetic material being selected. Applying Zeocin then favors cells that retain the construct, which can also support expression of an additional recombinant product.
This system is useful when researchers need to enrich genetically modified cells, establish stable cell lines, or support recombinant protein production. Because the source material describes its use in bacteria, yeast, and mammalian cells, it can fit experiments involving several types of biological hosts. Selection also helps verify that genetic transformation has occurred.
Growth under Zeocin indicates that cells in the culture carry and express a construct containing the resistance marker sufficiently to withstand the antibiotic. Researchers can therefore use continued growth as evidence supporting successful genetic transformation. In applications involving stable cell-line development or recombinant protein production, the selected surviving population becomes the basis for further work.