Cell Death
Cell Death - Related Videos
10:06
An Introduction to Cell Death
0 Views •
2023
Necrosis, apoptosis, and autophagic cell death are all manners in which cells can die, and these mechanisms can be induced by different stimuli, such as cell injury, low nutrient levels, or signaling proteins. Whereas necrosis is considered to be an “accidental” or unexpected form of cell death, evidence exists that apoptosis and autophagy are both programmed and “planned” by cells.In this introductory video, JoVE highlights key discoveries pertaining to cell death, including recent work done...
04:25
Neuronal Death Assay to Evaluate Cell Death in Ex Vivo Epilepsy Model
0 Views •
2025
This video demonstrates the technique of assessing neuronal death in rhinal cortex-hippocampus organotypic slices. Neuronal death occurs via evolving epileptic seizures — resembling in vivo epilepsy — and is detected through dual-staining of the dead cells using propidium iodide and immunofluorescence.
03:23
Cell Death Screening Using DAPI Staining: A Method for Rapid Screening of IR Induced Clonogenic Cell Death in Cancerous Cell Lines
0 Views •
2023
This video demonstrates the methodology to screen for major cell death profiles in ionizing radiation (IR) induced cancer cell lines using DAPI staining assay. The DAPI-stained nuclei of target cells undergoing cell death exhibit distinct morphologies and can be easily identified by fluorescence microscopy.
12:44
Use of LysoTracker to Detect Programmed Cell Death in Embryos and Differentiating Embryonic Stem Cells
0 Views •
Cited by 53 •
2012
We present a simple protocol to visualize regions of programmed cell death (PCD) in mouse embryos and differentiating embryonic stem (ES) cell cultures using a highly soluble dye called LysoTracker.
01:30
Overview of Cell Death
0 Views •
2023
Cell death is an essential process where the body gets rid of old or damaged cells. Cell proliferation and death need to be balanced, as an imbalance between the two may lead to cancer or autoimmune diseases. Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the 20th century...
FAQs
Caspases act as molecular executors that dismantle cellular components during apoptosis. This controlled breakdown also packages the remaining material, allowing it to be cleared rather than broadly released into surrounding tissue. The coordinated sequence helps remove selected cells while preserving tissue organization, making caspase activity central to regulated cell elimination.
Tissues remain functional when survival and elimination are appropriately balanced. Removing damaged or infected cells can protect tissue integrity, while excessive loss may disrupt normal architecture. Conversely, insufficient elimination can contribute to abnormal growth. This balance therefore connects cellular decisions with development, tissue maintenance, and disease.
Uncontrolled injury disrupts cellular membranes and releases intracellular contents, unlike apoptosis, which dismantles components and packages remnants for clearance. These different outcomes influence how surrounding tissue encounters dying-cell material. Comparing the two pathways helps explain why the form of cell death matters in biological maintenance and disease-related research.
Cell death research helps investigators examine how abnormal growth may arise when the balance between survival and elimination is disturbed. It also provides a framework for studying approaches intended to promote the removal of unwanted cells or prevent inappropriate survival. These questions connect cellular mechanisms with cancer biology and therapeutic development.
In neurodegeneration, researchers investigate how cell loss relates to disease, while infection studies consider the removal of infected cells and the consequences of that process. The same biological framework supports both areas, but the relevant cellular context differs. Examining these mechanisms can clarify how survival and elimination shape tissue outcomes.
Therapeutic strategies may be developed to promote or prevent particular forms of cell death, depending on the biological problem being studied. Promoting elimination may be relevant when unwanted cells persist, whereas prevention may be considered when cell loss contributes to tissue damage. Distinguishing regulated pathways from uncontrolled injury helps guide that objective.