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Use of LysoTracker to Detect Programmed Cell Death in Embryos and Differentiating Embryonic Stem Cells
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JoVE Journal Biology
Use of LysoTracker to Detect Programmed Cell Death in Embryos and Differentiating Embryonic Stem Cells

Use of LysoTracker to Detect Programmed Cell Death in Embryos and Differentiating Embryonic Stem Cells

DOI:
10.3791/4254-v

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12:44 min

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October 11, 2012

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, ,
1Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Keck School of Medicine,University of Southern California

Chapters

  • 00:05Title
  • 01:48LysoTracker Staining of Mouse Embryos
  • 04:53LysoTracker Staining of Differentiating ES Cultures
  • 06:51Mounting Samples for Imaging
  • 07:44Viewing LysoTracker-stained Samples and Image Analysis
  • 09:21LysoTracker Staining in Shh Null Embryos and Cultured Cells
  • 12:10Conclusion

Summary

Automatic Translation

Automatic Translation

We present a simple protocol to visualize regions of programmed cell death (PCD) in mouse embryos and differentiating embryonic stem (ES) cell cultures using a highly soluble dye called LysoTracker.

Tags

LysoTrackerProgrammed Cell DeathPCDEmbryosDifferentiating Embryonic Stem CellsApoptosisCaspase Enzyme CascadeMembrane BlebbingNuclear ShrinkingDNA FragmentationAutophagyImmune CellsNon-professional PhagocytesLysosomal Activity

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