We present a simple protocol to visualize regions of programmed cell death (PCD) in mouse embryos and differentiating embryonic stem (ES) cell cultures using a highly soluble dye called LysoTracker.
Fogel, J. L., Thein, T. Z. T., Mariani, F. V. Use of LysoTracker to Detect Programmed Cell Death in Embryos and Differentiating Embryonic Stem Cells. J. Vis. Exp. (68), e4254, doi:10.3791/4254 (2012).