Drought Tolerance Screening
Drought Tolerance Screening - Related Videos
06:35
Semi-High Throughput Screening for Potential Drought-tolerance in Lettuce (Lactuca sativa) Germplasm Collections
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Cited by 12 •
2015
This protocol was developed to screen a large germplasm collection of the leafy vegetable lettuce (Lactuca sativa L.) for drought-tolerance, in order to identify a small candidate pool of lettuce for use in physiological, molecular, and genetic studies to identify underlying drought-tolerance traits along with breeding programs.
02:41
Responses to Drought and Flooding
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2020
Water plays a significant role in the life cycle of plants. However, insufficient or excess of water can be detrimental and pose a serious threat to plants. Under normal conditions, water taken up by the plant evaporates from leaves and other parts in a process called transpiration. In times of drought stress, water that evaporates by transpiration far exceeds the water absorbed from the soil, causing plants to wilt. The general plant response to drought stress is the synthesis of hormone...
01:17
Salinity Tolerance of Alishewanella Species Isolated from Seawall Muddy Water
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2025
Begin with a culture of Alishewanella, a Gram-negative, salt-tolerant bacterium.Next, streak the culture onto agar plates containing increasing concentrations of sodium chloride to simulate varying levels of saline stress.Overlay each plate with a layer of unsolidified medium to prevent oxygen diffusion and maintain the culture in an oxygen-limited environment.Seal and incubate the plates at an optimum temperature to support bacterial growth.During incubation, the bacterium exhibits varying...
08:22
Remotely Supervised Transcranial Direct Current Stimulation: An Update on Safety and Tolerability
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Cited by 52 •
2017
This manuscript provides an updated remote supervision protocol that enables participation in transcranial direct current stimulation (tDCS) clinical trials while receiving treatment sessions from home. The protocol has been successfully piloted in both patients with multiple sclerosis and Parkinson's disease.
07:23
Assessment of Morphine-induced Hyperalgesia and Analgesic Tolerance in Mice Using Thermal and Mechanical Nociceptive Modalities
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Cited by 41 •
2014
We describe a protocol to examine the development of opioid-induced hyperalgesia and tolerance in mice. Based on the measurement of thermal and mechanical nociceptive responses of naïve and morphine-treated animals, it allows to quantify the increase in pain sensitivity (hyperalgesia) and decrease in analgesia (tolerance) associated with chronic opiate administration.
FAQs
Use a panel of indicators rather than a single endpoint. Germination reflects early establishment, biomass captures accumulated growth, leaf water status indicates plant hydration, and wilting shows visible loss of function. Evaluating these responses together helps distinguish genotypes that sustain development under limited water from those that only remain alive, producing a more informative biological profile.
Water conservation and continued physiological activity represent different drought-responsive strategies. A plant may retain more favorable leaf water status, indicating conservation, while another may continue growing or maintaining function despite reduced availability. Measuring leaf water status alongside biomass, germination, and wilting allows screening to capture these contrasting forms of tolerance rather than treating drought response as a single trait.
Recovery after rewatering adds a resilience dimension that measurements during stress cannot provide alone. Two genotypes may show comparable wilting or growth reduction while differing in their ability to resume performance once water returns. Including recovery helps identify plants whose drought response supports restoration, not merely short-term persistence, and strengthens interpretation of stress effects on development.
A controlled screen begins by imposing a defined period of limited water availability, then recording selected responses in the plant material being compared. Researchers can evaluate genotypes through germination, growth or biomass, leaf water status, wilting, and subsequent rewatering. This workflow links the imposed environmental condition to measurable outcomes and enables direct comparison of drought responses.
Results can reveal genotypes that maintain growth, function, or survival more effectively under limited water. Comparing these responses provides evidence for selecting resilient plant material and identifying drought-responsive traits relevant to breeding. The approach is especially useful when several indicators are considered together, because it can show whether a candidate performs well through water conservation, sustained activity, recovery, or a combination of responses.
The measurements connect environmental water limitation with changes in plant development and productivity. Germination, biomass, leaf water status, wilting, and recovery show how stress affects plants at different stages of performance. In biology, these outcomes help researchers examine drought adaptation mechanisms while also identifying responses associated with maintaining development and productivity under adverse conditions.