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Assessment of Morphine-induced Hyperalgesia and Analgesic Tolerance in Mice Using Thermal and Mechanical Nociceptive Modalities
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Assessment of Morphine-induced Hyperalgesia and Analgesic Tolerance in Mice Using Thermal and Mechanical Nociceptive Modalities

Assessment of Morphine-induced Hyperalgesia and Analgesic Tolerance in Mice Using Thermal and Mechanical Nociceptive Modalities

DOI:
10.3791/51264-v

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07:23 min

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July 29, 2014

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, , ,
1Biotechnology and Cellular Signalling, UMR 7242 CNRS,Université de Strasbourg

Chapters

  • 00:05Title
  • 01:24Tail Immersion Test (TIT)
  • 02:39Tail Pressure Test (TPT)
  • 03:31Acute Morphine-induced Analgesia
  • 04:42Chronic Morphine Treatment
  • 05:32Results: Morphine-induced Hyperalgesia and Analgesic Tolerance
  • 06:48Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a protocol to examine the development of opioid-induced hyperalgesia and tolerance in mice. Based on the measurement of thermal and mechanical nociceptive responses of naïve and morphine-treated animals, it allows to quantify the increase in pain sensitivity (hyperalgesia) and decrease in analgesia (tolerance) associated with chronic opiate administration.

Tags

Opioid-induced HyperalgesiaAnalgesic ToleranceMorphineThermal NociceptionMechanical NociceptionTail-immersion TestTail Pressure TestMouse Model

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