Epithelial Organoids
Epithelial Organoids - Related Videos
09:48
In Vitro Resident Memory CD8 T Cell Differentiation Using Epithelial Organoid-T Cell Co-culture System
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2026
To study CD8 T cell differentiation and function in vitro, CD8 T cells can be isolated from the mouse to be co-cultured long-term with preformed infected murine vaginal epithelial organoids. Here, we describe this process and assess the acquisition of resident memory T cell markers upon co-culture.
04:16
Prostate Organoid Assay: A Matrix Gel Ring-based Ex Vivo Culture Technique to Study the Differentiation Capacity of Prostate Epithelial Cells
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2023
In this video, we demonstrate the culturing of isolated mouse prostate epithelial cells using a matrix gel-ring based culture technique. This approach can provide complementary information about the differentiation capacity of prostate epithelial cells to form 3D glandular structures in response to genetic or pharmacological manipulation.
09:40
Organoid-Derived Epithelial Monolayer: A Clinically Relevant In Vitro Model for Intestinal Barrier Function
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Cited by 19 •
2021
Here, we describe the preparation of human organoid-derived intestinal epithelial monolayers for studying intestinal barrier function, permeability, and transport. As organoids represent original epithelial tissue response to external stimuli, these models combine the advantages of expandability of cell lines and the relevance and complexity of primary tissue.
10:30
Organoids as Model for Infectious Diseases: Culture of Human and Murine Stomach Organoids and Microinjection of Helicobacter Pylori
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Cited by 144 •
2015
Stem cell derived cultures harbor tremendous potential to model infectious diseases. Here, the culture of mouse and human gastric organoids derived from adult stem cells is described. The organoids are microinjected with the gastric pathogen Helicobacter pylori.
13:20
Culture and Imaging of Human Nasal Epithelial Organoids
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Cited by 3 •
2021
A detailed protocol is presented here to describe an in vitro organoid model from human nasal epithelial cells. The protocol has options for measurements requiring standard laboratory equipment, with additional possibilities for specialized equipment and software.
FAQs
Embedded in an extracellular matrix, epithelial stem or progenitor cells receive appropriate growth factors that support proliferation. As populations expand, they polarize and self-organize rather than remaining as an unstructured layer. This coordinated environment helps generate tissue-like architecture, including arrangements around a central lumen in vitro.
Polarity gives epithelial cells organized orientations within the developing structure, while a central lumen provides an internal tissue-like space. Together, these features help the model reproduce important epithelial characteristics beyond simple cell growth. They therefore support investigations of tissue maintenance, development, and barrier function in a controlled in vitro setting.
These models reproduce selected structural and functional features of epithelial tissues without requiring an intact animal system. Their in vitro format allows researchers to examine epithelial development, maintenance, barrier function, and disease mechanisms within organized tissue-like structures. Consequently, organoids can reduce reliance on animal systems while providing a biologically relevant experimental model.
A typical workflow begins with epithelial stem or progenitor cells, places them within an extracellular matrix, and supplies growth factors suited to their expansion and organization. The cells then proliferate, polarize, and form tissue-like structures, often around a lumen. These stages provide the structural basis for subsequent biological or biomedical analyses.
Researchers apply epithelial organoids when they need a tissue-like epithelial context for examining disease mechanisms or interactions between host tissue and pathogens. Because the cells organize into structures with epithelial features, the models can reveal how altered biological processes affect tissue behavior. This makes them useful for connecting cellular events with tissue-level outcomes.
Epithelial organoids provide organized in vitro models in which researchers can evaluate drug responses using tissue-like epithelial structures. Their applications also extend to personalized medicine, where organoid-based testing can help investigate responses relevant to an individual biological context. The same platform contributes to regenerative strategies by supporting research on epithelial tissue maintenance and repair.