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Immunology and Infection
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Organoids as Model for Infectious Diseases: Culture of Human and Murine Stomach Organoids and Microinjection of Helicobacter Pylori
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Immunology and Infection
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JoVE Journal Immunology and Infection
Organoids as Model for Infectious Diseases: Culture of Human and Murine Stomach Organoids and Microinjection of Helicobacter Pylori

Organoids as Model for Infectious Diseases: Culture of Human and Murine Stomach Organoids and Microinjection of Helicobacter Pylori

DOI:
10.3791/53359-v

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10:30 min

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November 12, 2015

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,
1Hubrecht Institute for Developmental Biology and Stem Cell Research,University Medical Centre Utrecht

Chapters

  • 00:05Title
  • 01:01Isolation and Seeding of Gastric Glands
  • 04:58Passage of Gastric Organoid Cultures
  • 06:55Microinjection of Organoid Cultures with Helicobacter pylori
  • 09:02Results: Organoid Formation and Infection with H. pylori
  • 09:48Conclusion

Summary

Automatic Translation

Automatic Translation

Stem cell derived cultures harbor tremendous potential to model infectious diseases. Here, the culture of mouse and human gastric organoids derived from adult stem cells is described. The organoids are microinjected with the gastric pathogen Helicobacter pylori.

Tags

Keywords: OrganoidsGastric OrganoidsHelicobacter PyloriHost-pathogen InteractionsStem CellsInfectious DiseasesCultureMicroinjectionImaging

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