Ex Vivo Podocyte Model
Ex Vivo Podocyte Model - Related Videos
04:19
Deriving Human Kidney Podocytes from Induced Pluripotent Stem Cells: A Procedure for Directed Differentiation of Mature Kidney Podocytes from Stem Cells
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2023
In this video, we describe the protocol to derive human kidney podocytes from induced pluripotent stem cells through cell differentiation under defined chemical conditions. The generated podocytes can be used for nephrotoxicity testing and disease modeling.
12:19
Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli
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Cited by 25 •
2015
Changes in the intracellular calcium levels in the podocytes are one of the most important means to control the filtration function of glomeruli. Here we explain a high-throughput approach that allows detection of real-time calcium handling and single ion channels activity in the podocytes of the freshly isolated glomeruli.
12:31
In Vivo Modeling of the Morbid Human Genome using Danio rerio
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Cited by 66 •
2013
Here, we present a systematic approach for developing physiologically relevant, sensitive and specific in vivo assays for interpreting variation in human pathology. Transient genetic manipulation via microinjection of WT and mutant human mRNA and morpholino (MO) antisense oligonucleotides harness the tractability of the developing zebrafish embryo to rapidly assay pathogenic mutations, especially, but not exclusively, in the context of human developmental disorders.
08:52
Generation of Patient-Derived Podocytes from Skin Biopsies
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Cited by 5 •
2023
This manuscript describes a two-step protocol to generate patient-specific podocytes from dermal fibroblasts via episomal reprogramming into human-induced pluripotent stem cells (hiPSCs) and subsequent differentiation into podocytes.
12:03
In Vivo Model for Testing Effect of Hypoxia on Tumor Metastasis
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Cited by 6 •
2016
This manuscript describes the development of an animal model that allows for the direct testing of the effects of tumor hypoxia on metastasis and the deciphering the mechanisms of its action. Although the experiments described here focus on Ewing sarcoma, a similar approach can be applied to other tumor types.
FAQs
Controlled exposure to defined stimuli lets researchers compare podocyte responses under consistent laboratory conditions. They can examine whether genetic, inflammatory, or drug-related stress is associated with altered morphology, cytoskeletal organization, or barrier function. This mechanistic comparison helps connect a specific challenge with cellular changes relevant to podocyte injury and proteinuria, rather than relying only on an organism-level outcome.
Cytoskeletal organization is an important cellular feature examined alongside barrier function. Changes in the podocyte cytoskeleton can be evaluated as a structural response to injury-related stimuli, while barrier measurements indicate whether that response accompanies altered filtration-barrier performance. Considering both readouts provides a more informative picture of podocyte responses than examining morphology alone.
The choice changes what the experiment can preserve and observe. Isolated podocytes support direct study of the cells themselves, whereas intact glomerular tissue retains aspects of the native renal environment. Comparing these formats can help researchers determine whether a finding reflects a cell-focused response or occurs within a more tissue-associated setting.
A basic workflow begins by selecting either isolated podocytes or intact glomerular tissue, then maintaining the material outside the organism under controlled conditions. Researchers expose the preparation to a defined stimulus and assess morphology, cytoskeletal organization, barrier function, or related injury responses. The same general sequence supports controlled comparisons across genetic, inflammatory, and drug-related challenges.
Measurements can show how podocytes change after a defined challenge, including effects on cell morphology, cytoskeletal organization, and barrier function. In disease-focused studies, these observations help characterize podocyte injury and changes associated with proteinuria. Because the conditions are controlled, the model also supports side-by-side evaluation of different stimuli or potential interventions.
It is useful when researchers need to investigate disease mechanisms or test a potential therapeutic intervention before moving to animal or clinical studies. The approach can connect cellular responses to genetic, inflammatory, or drug-related stress with kidney-relevant outcomes such as podocyte injury and proteinuria. Its value lies in providing an experimentally controlled stage for evaluation.