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Biology

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Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli
 

Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli

Article DOI: 10.3791/52850
June 27th, 2015

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Summary June 27th, 2015

Changes in the intracellular calcium levels in the podocytes are one of the most important means to control the filtration function of glomeruli. Here we explain a high-throughput approach that allows detection of real-time calcium handling and single ion channels activity in the podocytes of the freshly isolated glomeruli.

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