Fluoro-gold Labeling
Fluoro-gold Labeling - Related Videos
14:05
Retrograde Labeling of Retinal Ganglion Cells by Application of Fluoro-Gold on the Surface of Superior Colliculus
0 Views •
Cited by 40 •
2008
This video describes the method of retrograde labeling of RGC by applying fluoro-gold (FG) on the surface of superior colliculus (SC). Technique involves drilling the skull, aspirating the cortex, and applying gelatin sponge over entire dorsal surface of SC.
07:23
An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group
0 Views •
Cited by 5 •
2016
A detailed procedure for the synthesis of a 125I-labeled azide and the radiolabeling of dibenzocyclooctyne (DBCO)-group-conjugated, 13-nm-sized gold nanoparticles using a copper-free click reaction is described.
09:31
Gold Nanorod-assisted Optical Stimulation of Neuronal Cells
0 Views •
Cited by 9 •
2015
This protocol outlines how to use the transient heating associated with the optical absorption of gold nanorods to stimulate differentiation and intracellular calcium activity in neuronal cells. These results potentially open up new applications in neural prostheses and fundamental studies in neuroscience.
01:23
A Gold Staining Technique to Visualize Myelin Reduction in Stressed Mice
0 Views •
2025
Stress impairs oligodendrocyte function, disrupting the lipid-rich myelin sheath surrounding neuronal axons in the medial prefrontal cortex, a brain region regulating emotion.
07:39
High-Resolution Fluoro-Respirometry of Equine Skeletal Muscle
0 Views •
Cited by 2 •
2023
Horses have an exceptional aerobic exercise capacity, making equine skeletal muscle an important tissue for both the study of equine exercise physiology as well as mammalian mitochondrial physiology. This article describes techniques for the comprehensive assessment of mitochondrial function in equine skeletal muscle.
FAQs
Retrograde transport makes the tracer’s final location informative about projection direction. After Fluoro-gold is applied to a target region, axon terminals from projecting neurons take it up and move it toward their cell bodies. Fluorescence in those cell bodies therefore identifies neurons that send projections to the selected region, rather than neurons located there incidentally.
Axon-terminal uptake is central because it ties labeling to incoming projections at the selected site. The resulting signal is transported to neuronal cell bodies, allowing researchers to examine where those projecting neurons are located. This arrangement supports analysis of projection patterns across nervous-system regions, including pathways involving the brain or spinal cord.
Fluorescence microscopy provides the visual readout needed to locate tracer-containing cell bodies within nervous tissue. Researchers can examine these fluorescent neurons alongside anatomical or molecular markers, linking projection patterns with structural or molecular features. This combined analysis helps relate neural connectivity to the organization and characteristics of specific nervous-system regions.
An analysis typically begins by selecting a nervous-system region whose incoming projections are of interest. The tracer is applied there, and labeled cell bodies are subsequently located through fluorescence microscopy after transport within the pathway. Researchers can then compare the distribution of labeled neurons with anatomical or molecular markers to interpret connectivity.
Fluoro-gold labeling can identify neuronal populations that project to a chosen brain or spinal cord region and show how those populations are distributed. The resulting patterns provide information about neural-circuit organization and regional communication. Because labeled neurons can be evaluated with additional markers, the method also supports more detailed anatomical or molecular analyses.
In injury-related studies, researchers can use labeling patterns to examine changes in projections and connectivity within the nervous system. Comparing the distribution of labeled neurons across relevant conditions can indicate how communication between regions is altered. The technique therefore connects anatomical tracing with investigations of organization and pathway changes after nervous-system injury.