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Chapters
- 00:04Introduction
- 00:52Setting Up of the High‐Resolution Respirometer
- 02:04Measurement of Mitochondrial Membrane Potential Using Tetramethylrhodamine Methylester (TMRM)
- 02:54Measurement of ATP Production Using Magnesium Green (MgG)
- 03:46Measurement of Mitochondrial Production of ROSs Using Amplex UltraRed (AmR)
- 04:54Measurement of Mitochondrial Respiration
- 06:19Results: Characterization of Mitochondrial Physiology in Equine Skeletal Muscle
- 07:02Conclusion
Horses have an exceptional aerobic exercise capacity, making equine skeletal muscle an important tissue for both the study of equine exercise physiology as well as mammalian mitochondrial physiology. This article describes techniques for the comprehensive assessment of mitochondrial function in equine skeletal muscle.
