Fungal Susceptibility
Fungal Susceptibility - Related Videos
08:52
Live-cell Video Microscopy of Fungal Pathogen Phagocytosis
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Cited by 22 •
2013
We describe methods for live-cell video microscopy of Candida albicans phagocytosis by macrophages. These methods enable stage-specific analysis of macrophage migration, recognition, engulfment and phagosome maturation and reveal novel aspects of phagocytosis.
07:31
Microbial and Fungal Diversity - Student Protocol
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2019
Examining Microbial Colony Structures ExpandTo begin, collect your allocated culture plates and then remove the Parafilm and lid from one of them. Place the uncovered plate under a dissecting microscope and then observe the individual communities within each quadrant of the plate. As the colonies are identified, fill out the observed colony properties in the appropriate column in the table. Click Here to download Table 1 Then, repeat the observations for each of the other two bacterial species.
05:06
Microbial and Fungal Diversity - Concepts
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2019
Bacteria and fungi are two highly diverse groups of organisms that can have significant beneficial or detrimental impacts on human health. For this reason, it is important to understand and distinguish between individual species of these groups. As you will recall, biological taxonomists group organisms based on their phylogenetic relatedness. The three domains of life, Bacteria, Archaea, and Eukaryota segregate life into three distinct groupings. While all bacteria fall within the domain...
04:14
Establishing a Fungal-Bacterial Co-culture for Biofilm Formation
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2026
Source: Miquel Guennoc, C., et al. New Method for Qualitative Multi-scale Analysis of Bacterial Biofilms on Filamentous Fungal Colonies Using Confocal and Electron Microscopy. J. Vis. Exp. (2017)This video demonstrates the preparation of a fungal culture on treated cellophane membranes to support colony formation and radial growth. It then shows the cultivation and preparation of soil bacterial cells for interaction assays. Finally, it illustrates how bacterial cells adhere to the fungal colony...
02:24
A Technique to Quantify Fungal Infection in Zebrafish Larvae
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2025
This video demonstrates a method to quantify fungal infection in zebrafish larvae. Fungal spores are microinjected into the larval hindbrain ventricle, and the larvae are incubated to allow infection development. Infected larvae are harvested at defined intervals, and infection progress is assessed by enumerating colony-forming units.
FAQs
The MIC provides a standardized way to compare how much antifungal exposure is needed to prevent measurable fungal growth under specified test conditions. Because the value depends on both the organism and the drug, it should be interpreted as a result for that particular species or strain, agent, and testing setup, rather than as a universal property. This makes MIC data useful for comparing responses and supporting treatment decisions.
Variation reflects interaction among biological features of the fungus, the antifungal agent, and the experimental setup. Species or strains may respond differently, while changing test conditions can alter the measured growth response. Consequently, comparisons are most meaningful when the drug and testing conditions are defined consistently, allowing observed differences to be attributed more reliably to fungal biology.
Research on fungal susceptibility can use drug responses to examine cell-wall and membrane targets, because changes in growth after exposure provide clues about how chemical stress affects these structures. The results also help identify mechanisms that let fungi withstand treatment pressure. Linking susceptibility patterns with these targets connects a measured laboratory outcome to underlying cellular biology and survival.
Differences in susceptibility among strains can reveal more than immediate treatment response. They support surveillance for emerging resistance and help biologists compare how fungal populations tolerate chemical stress. In ecological contexts, this variation is relevant to survival because a fungus less affected by an antifungal agent may persist under exposure. Thus, susceptibility data connect clinical concerns with broader questions about adaptation and persistence.
A basic workflow exposes fungal cells to a series of defined antifungal concentrations, then measures growth under the selected testing conditions. Investigators use the resulting pattern to determine the minimum inhibitory concentration, or MIC, and compare responses across drugs, species, or strains. Keeping concentrations and conditions defined is essential for making the measurements interpretable and comparable.
Researchers apply these measurements when selecting potentially effective therapies for fungal infections, monitoring emerging resistance, or comparing responses among strains. The same results can support biological studies of drug-sensitive cellular structures, including the cell wall and membrane. Their value lies in linking an experimentally measured growth response with a practical decision, a surveillance trend, or a question about how fungi survive chemical stress.