Fusion Peptide Delivery
Fusion Peptide Delivery - Related Videos
01:33
Anti-Tumor Effect of a Fusion Peptide Delivered by Engineered Salmonella typhimurium in a Mouse Model
0 Views •
2026
Begin with mice bearing solid tumors implanted subcutaneously in the thigh.Inject the mice via the tail vein with a non-virulent, engineered strain of Salmonella typhimurium.The bacteria circulate in the bloodstream and selectively accumulate in the hypoxic, immunosuppressed tumor microenvironment.They carry a plasmid encoding a therapeutic fusion peptide composed of a cell-penetrating peptide and the mitochondrial target domain of a pro-apoptotic protein, along with phage-derived lysis...
10:30
Delivery of Proteins, Peptides or Cell-impermeable Small Molecules into Live Cells by Incubation with the Endosomolytic Reagent dfTAT
0 Views •
Cited by 10 •
2015
We describe how to deliver proteins and cell-impermeable small molecules into cultured mammalian cells by a simple co-incubation protocol with a reagent that causes endocytic organelles to become leaky.
07:35
Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli
0 Views •
Cited by 48 •
2014
Elastin-like polypeptides are stimulus-responsive biopolymers with applications ranging from recombinant protein purification to drug delivery. This protocol describes the purification and characterization of elastin-like polypeptides and their peptide or protein fusions from Escherichia coli using their lower critical solution temperature phase transition behavior as a simple alternative to chromatography.
02:45
Nuclear Fusion
0 Views •
2020
The process of converting very light nuclei into heavier nuclei is also accompanied by the conversion of mass into large amounts of energy, a process called fusion. The principal source of energy in the sun is a net fusion reaction in which four hydrogen nuclei fuse and ultimately produce one helium nucleus and two positrons. A helium nucleus has a mass that is 0.7% less than that of four hydrogen nuclei; this lost mass is converted into energy during the fusion. This reaction produces about...
09:19
Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay
0 Views •
2012
We have developed a cell fusion assay that quantifies SNARE-mediated membrane fusion events by activated expression of β-galactosidase.
FAQs
Successful uptake depends on coordinated membrane association and cellular entry. A cell-penetrating or membrane-active sequence brings the construct into contact with the membrane and can promote internalization. In some designs, pH-dependent changes or membrane disruption help the cargo escape endosomes, increasing access to intracellular pathways rather than leaving it sequestered.
The delivery sequence and functional payload contribute different requirements. The peptide must support membrane interaction and uptake without causing unacceptable membrane disruption, while the attached cargo must remain usable after entry. Designs that exploit pH-dependent behavior may improve endosomal release, so stability, toxicity, targeting, and release efficiency must be considered together.
It offers a flexible, peptide-based route for transporting macromolecules while potentially avoiding some limitations associated with viral vectors and conventional formulations. However, that comparison does not eliminate development challenges. Fusion-peptide systems still require evaluation of intracellular release, stability, toxicity, and targeting, because efficient entry alone does not guarantee useful delivery to the intended cellular compartment.
Development begins by matching the peptide architecture to the intended cargo and biological objective. Researchers then need to consider membrane association, cellular uptake, and whether endosomal escape is needed for the experiment. Stability, toxicity, targeting, and release efficiency provide additional evaluation criteria, helping distinguish a construct that enters cells from one that delivers functional cargo.
Supported cargoes include proteins, nucleic acids, imaging agents, and other macromolecules. These systems can therefore serve both delivery and investigation goals, such as examining intracellular pathways, studying gene regulation, or probing disease mechanisms. The useful outcome depends on whether the cargo reaches the relevant intracellular location and remains available for the intended measurement or biological effect.
Biology research often requires access to molecules whose limited membrane permeability prevents direct entry into cells. Fusion-peptide systems address that experimental barrier while offering a nonviral strategy for manipulating or observing intracellular processes. Their relevance spans mechanistic studies of uptake and gene regulation, imaging-based investigations, and disease-mechanism research, provided delivery performance and cellular effects are interpreted together.