In Utero Injection
In Utero Injection - Related Videos
19:04
In Utero Intraventricular Injection and Electroporation of E16 Rat Embryos
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Cited by 13 •
2007
In-utero in-vivo injection and electroporation of the embryonic rat neocortex provides a powerful tool for the manipulation of individual progenitors ...
26:17
In Utero Intraventricular Injection and Electroporation of E15 Mouse Embryos
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Cited by 52 •
2007
In-utero in-vivo injection and electroporation of the embryonic mouse neocortex provides a powerful tool for the manipulation of individual progenitors ...
08:05
Murine In Utero Electroporation
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2023
In utero electroporation is an important technique for studying the molecular mechanisms that guide the proliferation, differentiation, migration, and maturation of cells during neural development. Electroporation enables the rapid and targeted delivery of material into cells by utilizing electrical pulses to create transient pores in cell membranes. Although electroporation has traditionally been used in in vitro studies, scientific advancements have now broadened its utilization to intact...
09:11
A Mouse Model of in Utero Transplantation
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Cited by 26 •
2011
The mouse model of in utero transplantation is a versatile tool that can be used to study the potential clinical applications of stem cell transplantation and gene therapy in the fetus. In this protocol, we present a general approach to performing this...
09:33
Utero-tubal Embryo Transfer and Vasectomy in the Mouse Model
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Cited by 28 •
2014
Utero-tubal embryo transfer uses the utero-tubal junction as a barrier to prevent the embryo outflow that may occur when performing uterine transfer. Vasectomized males are required to obtain pseudopregnant recipients for embryo transfer. Both techniques are discussed.
FAQs
The target compartment determines where the introduced material first acts and what biological response can be examined. Investigators may direct substances to the amniotic cavity, placenta, or fetal tissues depending on whether they are studying exposure, placental biology, or tissue-specific effects. This choice helps connect delivery location with developmental, therapeutic, or disease-related outcomes.
Prenatal biology changes across developmental windows, so the same substance may produce different effects depending on when it is delivered. Studying a defined window allows researchers to examine treatment activity, tissue formation, or disease mechanisms in relation to ongoing development. It also helps evaluate whether effects occur early enough to influence a prenatal biological process.
Interpretation depends on the delivered substance, its destination, the developmental stage, and the degree of experimental control maintained during delivery. Cells, nucleic acids, drugs, and tracers provide different kinds of biological information, while targeting distinct compartments changes the context of exposure. Considering these variables helps distinguish delivery effects from the response being studied.
The technique provides a way to examine how nucleic acids or cells behave when introduced before birth and to assess their biological effects during development. Researchers can investigate whether a prenatal intervention influences tissue formation or disease mechanisms, while also examining safety under controlled conditions. These observations can inform evaluation of therapeutic strategies before birth.
A study requires selecting the substance and intended target, establishing controlled experimental conditions, and introducing the material with a fine needle. Imaging may guide the needle when precise placement is needed. Researchers then assess the resulting biological effects in relation to the targeted compartment and developmental window, allowing delivery conditions to be linked with experimental outcomes.
In biology, the approach can reveal how prenatal treatments, cells, nucleic acids, drugs, or tracers affect embryonic development and tissue formation. It can also help investigate disease mechanisms and examine responses during critical developmental windows. By connecting a defined delivery site with observed effects, studies can generate information about both biological processes and prospective prenatal interventions.