Intravenous Microinjection
Intravenous Microinjection - Related Videos
04:18
Intravenous Microinjection of Nephrotoxins into Zebrafish Embryo: A Technique to Deliver Nephrotoxic Agents into the Bloodstream of Zebrafish Embryo
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2023
This video demonstrates the intravenous microinjection of nephrotoxins into the tail vein of a zebrafish embryo to study their chemical effects on renal function.
15:55
Intravenous Microinjections of Zebrafish Larvae to Study Acute Kidney Injury
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Cited by 83 •
2010
We describe a technique of microinjecting the aminoglycoside, gentamicin, into 2 days post-fetilization (dpf) zebrafish larvae to induce acute kidney injury (AKI). We also describe a method for whole mount immunohistochemistry, plastic embedding and sectioning of zebrafish larvae to visualize the AKI mediated damage.
07:22
Discontinuing Intravenous Fluids and a Peripheral Intravenous Line
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2023
Source: Madeline Lassche, MSNEd, RN and Katie Baraki, MSN, RN, College of Nursing, University of Utah, UT Intravenous (IV) fluid administration and peripheral IV catheters (PIVs) may be discontinued for a number of reasons. The most common reason for discontinuing IV fluids is that the patient has returned to normal body fluid volume (euvolemia) and is able to maintain adequate oral fluid intake or is being discharged from the hospital. In addition, the Centers for Disease Control Guidelines...
08:12
Zebrafish Microinjection Techniques
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2023
One of the major advantages to working with zebrafish (Danio rerio) is that their genetics can be easily manipulated by microinjection of early stage embryos. Using this technique, solutions containing genetic material or knockdown constructs are delivered into the blastomeres: the embryonic cells sitting atop the yolk of the newly fertilized egg. Delivery into the cytoplasm is achieved either through direct injection into the blastomere, or via natural cytoplasmic movements that occur after a...
11:39
Peripheral Intravenous Catheter Insertion
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2023
Source: Madeline Lassche, MSNEd, RN and Katie Baraki, MSN, RN, College of Nursing, University of Utah, UT The purpose of peripheral intravenous catheter (PIV) insertion is to infuse medications, perform intravenous (IV) fluid therapy, or inject radioactive tracers for special examination procedures. Placing a PIV is an invasive procedure and requires the use of an aseptic, no-touch technique. Common IV venipuncture sites are the arms and hands in adults and the feet in children. According to...
FAQs
Controlled pressure allows the defined dose to be dispensed slowly after the needle enters the vein. This helps manage how the material enters systemic circulation while reducing the likelihood of leakage and local tissue damage. In biological experiments, maintaining this control supports more consistent delivery of drugs, tracers, or other test materials between animal-model investigations.
A precisely measured volume establishes a defined dose for each experiment, making subsequent observations easier to relate to the administered material. This is particularly important when researchers examine pharmacokinetics, vascular distribution, disease mechanisms, or treatment responses. Consistent dosing helps distinguish biological effects from differences caused by variable delivery amounts.
Intravenous microinjection places the experimental material directly into a blood vessel rather than depositing it primarily at a local tissue site. The material can therefore enter systemic circulation efficiently, supporting investigations of distribution throughout the vascular system. Careful needle guidance and dispensing remain important because leakage or tissue injury can compromise the intended delivery.
Needle placement, the measured volume, and the pressure and speed used for dispensing can all influence delivery. The needle must be guided into the vein, and the dose should be released slowly under controlled pressure. Managing these factors helps limit leakage and local tissue damage while preserving the intended entry of the test material into circulation.
The procedure begins by preparing a defined dose of the selected experimental material. A fine needle is then guided into a blood vessel, after which the material is dispensed slowly under controlled pressure. This sequence is designed to support entry into systemic circulation and to limit leakage or local tissue damage during delivery in an animal model.
The technique can deliver drugs, molecular tracers, and other test materials used in biological experiments. Researchers select the material according to the question being studied, such as tracking vascular distribution, examining pharmacokinetics, or evaluating treatment responses. The method therefore accommodates both substances intended to produce an experimental effect and materials used to follow biological movement.
In animal models, intravenous microinjection provides a controlled way to introduce experimental substances into systemic circulation. This supports studies of how materials distribute through the vascular system, how drug exposure relates to responses, and how disease mechanisms or treatments can be investigated. The controlled dose and delivery route make it useful across several experimental biology applications.