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Intravenous Microinjections of Zebrafish Larvae to Study Acute Kidney Injury
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Intravenous Microinjections of Zebrafish Larvae to Study Acute Kidney Injury

Article DOI: 10.3791/2079-v • 15:56 min • August 4th, 2010
August 4th, 2010 •
1, 2, 3, 1
1Department of Developmental Biology, University of Pittsburgh, 2Department of Biological Sciences, University of Pittsburgh, 3Department of Medicine and Genetics, Harvard Medical School

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Summary
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We describe a technique of microinjecting the aminoglycoside, gentamicin, into 2 days post-fetilization (dpf) zebrafish larvae to induce acute kidney injury (AKI). We also describe a method for whole mount immunohistochemistry, plastic embedding and sectioning of zebrafish larvae to visualize the AKI mediated damage.

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Intravenous Microinjections Zebrafish Larvae Acute Kidney Injury Gentamicin Nephrotoxicant Bloodstream Fish Per Hour Experimental Studies Angiography Protocol Kidney Function Fixation Immunohistochemistry Kidney Tubule Marker Plastic Embedding Sectioning
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