Klh Emulsion
Klh Emulsion - Related Videos
04:08
Preparing an Antigen-Adjuvant Emulsion to Induce Autoimmune Encephalomyelitis
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2025
This video demonstrates an efficient homogenization technique to generate an antigen-adjuvant emulsion to induce experimental autoimmune encephalomyelitis (EAE) in a murine model. The emulsion contains an autoantigen — myelin oligodendrocyte glycoprotein (MOG) — in the water phase, surrounded by a continuous oil phase comprising an inactivated pathogenic bacteria. The emulsion ensures a sustained release of antigens for a prolonged immune response.
08:35
Minimum Burning Pressures of Water-based Emulsion Explosives
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2017
We present an apparatus based on hot-wire ignition in a pressurized enclosure and an associated methodology to measure the minimum pressure required to induce sustained combustion in water-based emulsion explosives. This method improves product characterization to allow one to use them more safely during pumping and mixing operations.
10:52
Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets
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Cited by 19 •
2016
The assembly and positioning of the mitotic spindle depend on the combined forces generated by microtubule dynamics, motor proteins and cross-linkers. Here we present our recently developed methods in which the geometrical confinement of spherical emulsion droplets is used for the bottom-up reconstitution of basic mitotic spindles.
12:48
PLGA Nanoparticles Formed by Single- or Double-emulsion with Vitamin E-TPGS
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Cited by 211 •
2013
We describe the production and characterization of nanoparticles and microparticles composed of poly(lactic-co-glycolic acid) using vitamin E-TPGS as an emulsifier. By varying formulation parameters such as the concentration of emulsifier, it is possible to produce nanoparticles with mean diameters ranging from 220 nm to 1.98 µm.
08:58
Double Emulsion Generation Using a Polydimethylsiloxane (PDMS) Co-axial Flow Focus Device
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Cited by 8 •
2015
Microfluidic double emulsions generation typically involves devices with patterned wettability or custom-fabricated glass components. Here we describe the fabrication and testing of an all polydimethylsiloxane (PDMS) double emulsion generator that does not require surface treatment or complicated fabrication processes, and is capable of producing double emulsions down to 14 µm.
FAQs
The emulsion shapes how the antigen is retained and presented after delivery. Vigorous mixing produces a stable suspension, either water-in-oil or oil-in-water, that can help keep KLH at the injection site while the adjuvant activates innate immune pathways. These effects support antigen uptake and presentation, strengthening the conditions required for an antigen-specific immune response.
KLH serves as the highly immunogenic carrier protein, providing antigenic material that can stimulate adaptive immunity. The adjuvant contributes immune stimulation through innate pathways and helps the formulation retain antigen after injection. Combining these roles can promote both antigen uptake and presentation, which supports the later development of antigen-specific humoral and cellular responses.
Vigorous mixing disperses the KLH-containing phase throughout the adjuvant and helps create a stable suspension. The resulting formulation may be water-in-oil or oil-in-water, depending on how the phases are organized. Stability matters because controlled dispersion and delivery influence antigen retention at the injection site and the subsequent interaction between antigen and immune cells.
Preparation begins by combining KLH with an adjuvant, followed by vigorous mixing to disperse the protein and form a stable emulsion. The formulation is then used for delivery in an immunization setting. Attention to controlled formulation and delivery is important because these features affect antigen retention, innate immune activation, and the quality of antigen presentation.
Researchers may select a KLH emulsion when they need to immunize laboratory animals and generate antibodies directed against a target antigen associated with the carrier. The formulation supports immune stimulation through antigen retention, innate pathway activation, and antigen presentation. Resulting antigen-specific antibodies can then support immunoassays and other investigations of immune reactivity.
KLH emulsion studies can examine both antibody-mediated, or humoral, immunity and cellular immune responses. Its formulation promotes antigen uptake and presentation while the adjuvant stimulates innate pathways, creating a context for evaluating how these immune branches respond. Researchers also apply the system to investigate immune tolerance, immune reactivity, and vaccine-related questions.