Lesion-specific Detection
Lesion-specific Detection - Related Videos
12:45
Stereotaxic Surgery for Excitotoxic Lesion of Specific Brain Areas in the Adult Rat
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Cited by 22 •
2012
Targeted ablation of specific brain region(s) by infusion of an excitotoxin using stereotaxic coordinates is described. This technique could also be adapted for infusion of other chemicals into the rat brain.
15:01
Quantitative, Real-time Analysis of Base Excision Repair Activity in Cell Lysates Utilizing Lesion-specific Molecular Beacons
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Cited by 31 •
2012
We describe a method for the quantitative, real-time measurement of DNA glycosylase and AP endonuclease activities in cell nuclear lysates. The assay yields rates of DNA Repair activity amenable to kinetic analysis and is adaptable for quantification of DNA Repair activity in tissue and tumor lysates or with purified proteins.
08:16
Comparative Lesions Analysis Through a Targeted Sequencing Approach
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2019
This article describes a method to identify clonal and subclonal alterations among different specimens from a given patient. Although the experiments described here focus on a specific tumor type, the approach is broadly applicable to other solid tumors.
03:20
Nested-PCR to Detect a Specific Viral Genomic Sequence
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2025
This video describes nested polymerase chain reaction, a technique that consists of two sequential PCR amplification processes using two primer sets. The first set of primers is intended to anneal to sequences upstream of the second set, resulting in selective amplification of specific gene sequences. This PCR is more sensitive and specific than a normal PCR and is widely used as a detection technique for various diseases.
06:43
Quantitative Analysis and Characterization of Atherosclerotic Lesions in the Murine Aortic Sinus
0 Views •
Cited by 51 •
2013
We describe procedures to quantify and characterize atherosclerotic lesions in mouse models using precision sectioning of the aortic sinus and ascending aorta combined with histochemical and immunohistochemical analysis.
FAQs
Specificity comes from matching a recognition element to a feature characteristic of the lesion rather than to the surrounding sample. A structural epitope, altered sequence, or chemical modification can serve as that feature. When the matching antibody, nucleic acid probe, or repair enzyme binds, the assay focuses its signal on the selected abnormality.
The recognition element determines which molecular evidence becomes visible. Antibodies can bind structural epitopes, nucleic acid probes can identify altered sequences, and repair enzymes can recognize particular DNA damage features. This choice links the assay to the lesion’s chemistry or structure, helping researchers distinguish one abnormality from normal material and interpret the resulting signal more specifically.
Compared with assays that measure overall damage, lesion-specific detection provides information about damage identity as well as amount. A broad damage assay may indicate that abnormality is present, whereas a lesion-directed signal can show whether a defined structural, sequence, or chemical feature is present. This distinction matters when the research question concerns a particular lesion rather than total damage.
A basic workflow begins by selecting the lesion feature and a compatible recognition element, then applying that element to the complex biological sample. The bound feature is visualized or measured using microscopy, electrophoresis, or immunoassay. The resulting signal is interpreted as evidence of the selected lesion, allowing its location or burden to be examined instead of treating all sample material as equivalent.
Microscopy is useful when the study concerns where a lesion occurs within a sample, because it can support mapping of DNA damage or comparison of diseased and normal tissue. Electrophoresis and immunoassay provide alternative ways to generate measurable lesion-associated signals. The selected readout therefore depends on whether the study emphasizes distribution, distinction, or quantity.
In biology, these measurements can quantify lesion burden and evaluate repair or treatment responses. Comparative measurements can show whether the amount of a defined abnormality changes between diseased and normal material or after an intervention. Because the assay targets a specified lesion, those comparisons are more informative than a measure of total, unidentified damage.