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Stereotaxic Surgery for Excitotoxic Lesion of Specific Brain Areas in the Adult Rat
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JoVE Journal Neuroscience
Stereotaxic Surgery for Excitotoxic Lesion of Specific Brain Areas in the Adult Rat

Stereotaxic Surgery for Excitotoxic Lesion of Specific Brain Areas in the Adult Rat

DOI:
10.3791/4079-v

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12:45 min

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July 19, 2012

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, , ,
1Helen Wills Neuroscience Institute,University of California Berkeley, 2Office of Laboratory Animal Care,University of California Berkeley, 3McGovern Institute for Brain Research & The Department of Brain and Cognitive Science,Massachusetts Institute of Technology, 4Integrative Biology Department,University of California Berkeley

Chapters

  • 00:05Title
  • 01:11Preparation of the Pump and Stereotax
  • 03:26Preparing the Rat for Surgery
  • 04:36Creating the Surgical Window
  • 05:16Leveling the Skull and Creating the Pilot Hole
  • 07:13Infusion of Neurotoxin
  • 09:03Closing the Incision
  • 10:00Results: Effects of the Cytotoxic Lesion
  • 12:17Conclusion

Summary

Automatic Translation

Automatic Translation

Targeted ablation of specific brain region(s) by infusion of an excitotoxin using stereotaxic coordinates is described. This technique could also be adapted for infusion of other chemicals into the rat brain.

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Stereotaxic SurgeryExcitotoxic LesionSpecific Brain AreasAdult RatLocalized AblationElectrolytic LesionsInducible Genetic TechniquesCell-type Specific PromotersStereotaxic InfusionExcitotoxinN-methyl-D-aspartate (NMDA)Basolateral Amygdala Complex

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