Lung Extraction
Lung Extraction - Related Videos
05:38
RNA Extraction Assay: A Method to Extract RNA from miRNA Transfected Lung Cancer Cells
0 Views •
2023
This video describes the RNA extraction protocol from miRNA transfected lung cancer cells. This approach can be used to identify a combinatorial miRNA treatment activity in halting the cell cycle and angiogenesis.
03:03
Pig Bronchiolar Tissue Extraction: A Procedure to Dissect Bronchiolar Tissue Sections From Porcine Lungs
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2025
In this video, we describe a surgical procedure to extract bronchiolar tissues from harvested pig lung. The ex vivo bronchiolar tissue could be used to develop infection models for studying chronic lung infections.
12:50
Procedure for Lung Engineering
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Cited by 43 •
2011
We have developed a decellularized lung extracellular matrix and novel biomimetic bioreactor that can be used to generate functional lung tissue. By seeding cells into the matrix and culturing in the bioreactor, we generate tissue that demonstrates effective gas exchange when transplanted in vivo for short periods of time.
07:06
A Simple Method of Mouse Lung Intubation
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Cited by 50 •
2013
This paper describes a striaghforward and efficient method of intubating mice for pulmonary function measurements or pulmonary instillation, that allows the mice to recover and be studied at later times. The procedure involves an inexpensive fiberoptic light source that directly illuminates the trachea.
03:59
miRNA Extraction: A Method to Extract miRNA from Plasma Sample
0 Views •
2023
MicroRNAs (miRNAs) are tissue specific, small, non-coding RNAs regulating gene expression, and could represent ideal candidates for early detection of lung cancer. In the featured protocol, we will isolate microRNA from patient blood samples for early lung cancer screening.
FAQs
Maintaining tissue architecture keeps spatial relationships among lung structures available for examination. This is important because histology and imaging depend on recognizable organization rather than isolated or severely damaged fragments. Careful handling therefore allows researchers to relate whole-organ features to cellular findings and pathological changes, strengthening interpretation across different types of analysis.
Controlled separation limits damage caused by forceful handling and helps prevent material from surrounding structures from contaminating the sample. These safeguards improve the reliability of later observations, especially when researchers compare anatomy, tissue condition, or molecular results among specimens. Consistent sample quality makes differences more likely to reflect biology rather than variation introduced during collection.
An extracted lung can support examination at several linked levels, from whole-organ anatomy to tissue structure, cellular features, and molecular findings. This connection helps researchers interpret respiratory conditions more comprehensively. For example, an anatomical observation can be evaluated alongside histological, imaging, or molecular results instead of being considered in isolation.
The workflow begins by accessing the thoracic cavity, followed by carefully separating the lungs from surrounding structures. Handling must preserve the tissue and minimize damage or contamination. After removal, the sample can be directed toward preservation, anatomical assessment, histology, imaging, or molecular analysis, depending on the scientific question and intended downstream use.
Lung samples can be preserved for later examination or processed for histology, imaging, and molecular studies. These approaches provide complementary information: histology examines tissue organization, imaging documents structural features, and molecular studies investigate biological characteristics at the molecular level. Using more than one approach can support a broader assessment of respiratory tissue.
The procedure is useful when researchers need direct access to lung tissue for anatomical assessment or investigation of respiratory disease. In experimental biology, standardized extraction supports comparisons among samples and helps relate organ-level observations to cellular, physiological, and pathological findings. Its value therefore extends from basic structural studies to broader analyses of disease-related changes.