Mirna Recruitment
Mirna Recruitment - Related Videos
03:59
miRNA Extraction: A Method to Extract miRNA from Plasma Sample
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2023
MicroRNAs (miRNAs) are tissue specific, small, non-coding RNAs regulating gene expression, and could represent ideal candidates for early detection of lung cancer. In the featured protocol, we will isolate microRNA from patient blood samples for early lung cancer screening.
04:33
Circulating miRNA Extraction: A Method to Isolate miRNA from Plasma Samples Via Organic Extraction and Small RNA Enrichment
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2023
This video describes the extraction of circulating microRNA from plasma samples of colorectal cancer patients using organic extraction followed by glass-fiber column enrichment. miRNAs are stable in biological fluids such as serum and plasma, which renders them ideal circulating biomarkers for cancer diagnosis, prognosis, and treatment decision-making and monitoring. This method will help identify new circulating biomarkers capable of predicting clinical outcomes in patients.
04:00
Modelling Intratumoral Treatment with miRNA Inhibitors: A Method to Analyze the Effect of miRNA Inhibitors on Tumor Growth in Murine Model
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2023
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05:38
RNA Extraction Assay: A Method to Extract RNA from miRNA Transfected Lung Cancer Cells
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This video describes the RNA extraction protocol from miRNA transfected lung cancer cells. This approach can be used to identify a combinatorial miRNA treatment activity in halting the cell cycle and angiogenesis.
04:28
Lung Tumor Cell Recruitment Assay
0 Views •
2019
This manuscript describes a method to quantify tumor cell accumulation in the lungs in an animal model of tumor metastasis.
FAQs
Argonaute loading places a mature miRNA within the silencing complex, creating the molecular component that guides target recognition. Without this association, the miRNA cannot be positioned to use its sequence information against a messenger RNA. Consequently, recruitment links the availability of mature miRNA to selective post-transcriptional control of gene expression.
The seed region provides the principal sequence-matching information used during target recognition. Complementary base pairing in this region helps the silencing complex distinguish messenger RNAs that carry an appropriate target sequence from other cellular RNAs. This selectivity supports focused regulation of particular transcripts rather than indiscriminate suppression across the cell.
Once recognition occurs, regulation can produce translational repression or messenger RNA degradation. Both outcomes reduce gene expression, but they have distinct consequences for the target RNA and its use in protein production. Distinguishing these results helps researchers describe whether recruitment primarily limits translation from an existing transcript or reduces the transcript itself.
By controlling gene expression after transcription, miRNA recruitment can influence the levels of proteins involved in development, differentiation, metabolism, and responses to cellular stress. Studying recruitment in these settings helps connect specific RNA-targeting events with broader changes in cell state, offering a way to investigate how post-transcriptional regulation contributes to biology.
Research on miRNA recruitment can clarify how altered post-transcriptional regulation contributes to disease mechanisms. Because recruitment affects which messenger RNAs undergo repression or degradation, its patterns may also help identify molecular changes associated with disease. These findings support investigation of miRNAs and their targets as potential biomarkers for biological or pathological states.
Understanding the steps that connect mature miRNAs, Argonaute proteins, target recognition, and gene-silencing outcomes can guide strategies designed to modulate gene expression. Researchers can use this framework to consider whether changing recruitment or its downstream effects might alter disease-relevant transcripts, making the process relevant to therapeutic development as well as basic Biology.