Mrna Microinjection
Mrna Microinjection - Related Videos
11:32
Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection
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Cited by 31 •
2010
Here we describe an assay that employs the power of microinjection coupled with fluorescent in situ hybridization in order to accurately measure the nuclear export kinetics of mRNA in mammalian somatic cells.
11:33
Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.
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Cited by 86 •
2009
Microinjection is a well-established and effective method for introducing foreign substances into fertilized zebrafish embryos. Here, we demonstrate a robust microinjection technique for performing mRNA overexpression, and morpholino oligonucleotide gene knockdown studies in zebrafish.
08:12
Zebrafish Microinjection Techniques
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2023
One of the major advantages to working with zebrafish (Danio rerio) is that their genetics can be easily manipulated by microinjection of early stage embryos. Using this technique, solutions containing genetic material or knockdown constructs are delivered into the blastomeres: the embryonic cells sitting atop the yolk of the newly fertilized egg. Delivery into the cytoplasm is achieved either through direct injection into the blastomere, or via natural cytoplasmic movements that occur after a...
08:33
Cell-Free In Vitro mRNA Translation In Drosophila Lysates
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2026
Here, we provide details on how to prepare lysates and perform in vitro translation using Drosophila embryos.
02:22
Regulated mRNA Transport
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2020
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...
FAQs
After the transcript reaches the cytoplasm, cellular ribosomes translate it into the selected protein. Expression remains temporary because the injected RNA is naturally degraded rather than maintained indefinitely. This combination lets researchers examine protein activity during a defined period and compare outcomes produced by different expression timings or controlled amounts of transcript.
The target system determines where the mRNA becomes available for translation. Researchers may inject the cytoplasm of a cell or work with a fertilized egg, depending on the biological question. This flexibility supports studies ranging from protein production in individual cells to gene-function analysis during the earliest stages of development.
mRNA microinjection supplies a transcript that ribosomes can translate directly, whereas the approach described here does not introduce DNA into the genome. As a result, expression is transient and linked to RNA persistence rather than long-term genomic maintenance. That distinction is useful when researchers need temporary protein production without genomic introduction.
A researcher prepares an mRNA solution, loads it into a fine glass needle, positions the needle within the selected cell or fertilized egg, and delivers the transcript into the cytoplasm. The resulting protein production can then be examined during the period before the RNA is degraded, with timing and dose treated as controlled experimental variables.
The method is applicable to embryos, oocytes, and cultured cells. In embryos and fertilized eggs, it can support investigations of early development and gene function. In oocytes or cultured cells, transient production of a chosen protein can help researchers examine protein activity or cell signaling in a defined experimental setting.
This approach can test how a selected protein influences gene function, early development, cell signaling, or other cellular activity. Because researchers can control when and how much transcript enters the system, they can relate observed biological effects to temporary protein expression. The resulting experiments provide a focused way to investigate protein function without relying on permanent genomic changes.