Sgrna Complex
Sgrna Complex - Related Videos
02:15
Nanoblade-Based Delivery of Nucleic Acid Cargo: A Technique to Deliver Cas9-sgRNA Complex to Target Cells via Virus-Like Particles
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2025
This video demonstrates a technique for nanoblade-based delivery of Cas9-sgRNA ribonucleoprotein complex inside target cells for genome editing. Nanoblades are virus-like particles devoid of the ability to multiply and infect neighboring cells, therefore are very useful for rapid and dose-dependent transportation of bio- and nanomaterials.
09:23
Delivery of the Cas9/sgRNA Ribonucleoprotein Complex in Immortalized and Primary Cells via Virus-like Particles ("Nanoblades")
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Cited by 6 •
2021
We have developed a simple and inexpensive protocol to load Cas9/single-guide RNA (sgRNA) ribonucleoprotein complexes within virus-like particles. These particles, called "Nanoblades", allow efficient delivery of the Cas9/sgRNA complex in immortalized and primary cells as well as in vivo.
04:51
High-Efficiency Gene Disruption in Primary Bone Marrow-Derived Macrophages Using Electroporated Cas9-sgRNA Complexes
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2023
This protocol describes the procedure for genome editing in mouse bone marrow-derived macrophages using Cas9-sgRNA ribonucleoprotein complexes assembled in vitro and delivered by electroporation.
06:12
Production of Genetically Engineered Golden Syrian Hamsters by Pronuclear Injection of the CRISPR/Cas9 Complex
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Cited by 24 •
2018
Pronuclear (PN) injection of the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein-9 nuclease (CRISPR/Cas9) system is a highly efficient method for producing genetically engineered golden Syrian hamsters. Herein, we describe the detailed PN injection protocol for the production of gene knockout hamsters with the CRISPR/Cas9 system.
08:42
Coordination Chemistry Complexes
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2023
Source: Laboratory of Dr. Neal Abrams — SUNY College of Environmental Science and Forestry Transition metals are found everywhere from vitamin supplements to electroplating baths. Transition metals also make up the pigments in many paints and compose all minerals. Typically, transition metals are found in the cationic form since they readily oxidize, or lose electrons, and are surrounded by electron donors called ligands. These ligands do not form ionic or covalent bonds with the metal center,...
FAQs
The guide region determines which DNA sequence the complex recognizes through complementary base pairing, whereas the scaffold provides the structural interaction required for nuclease binding and activation. This division of roles allows one sgRNA framework to direct a nuclease toward different target sequences by changing the guide region while preserving the scaffold that supports assembly.
A compatible protospacer-adjacent motif, or PAM, provides a required sequence context near the intended DNA target. Even when the guide can base-pair with a complementary region, the targeting process depends on the appropriate PAM being present. PAM availability therefore constrains which genomic sites an sgRNA complex can recognize and potentially cleave.
Targeting efficiency and specificity depend strongly on sgRNA design and delivery. The guide must support recognition of the intended DNA region, while the assembled complex must reach the relevant biological context in an effective form. These factors influence whether the complex produces the desired localized cleavage, supports genome editing, or yields less predictable experimental results.
A typical workflow begins by designing a guide sequence complementary to the selected DNA region and checking for a compatible PAM. The guide is then combined with its scaffold and associated nuclease before delivery into the experimental system. Researchers use the resulting complex to promote localized DNA cleavage and examine the resulting editing or gene-function outcome.
Researchers can use an sgRNA complex when they need to connect a selected DNA target with a biological consequence. Localized cleavage can support targeted genome editing, allowing gene-function studies to examine outcomes associated with a chosen genomic region. The approach is especially relevant when sequence-directed intervention is preferred over changes applied broadly across the genome.
The same targeting principle can be applied to different biological objectives. When the associated nuclease promotes localized DNA cleavage, the complex supports targeted genome editing and gene-function analysis. In other experimental contexts, sgRNA complexes contribute to regulation of gene expression. Thus, guide design and delivery help determine whether the emphasis is DNA alteration or expression control.