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Production of Genetically Engineered Golden Syrian Hamsters by Pronuclear Injection of the CRISPR/Cas9 Complex
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1Department of Animal, Dairy, and Veterinary Sciences,Utah State University, 2National Centre for International Research in Cell and Gene Therapy, Sino-British Research Centre, School of Basic Medical Sciences,Zhengzhou University, 3Department of Animal Science Division of Applied Life Science (BK21 Plus),Gyeongsang National University, 4Institute of Agriculture and Life Science,Gyeongsang National University, 5Centre for Molecular Oncology, Barts Cancer Institute,Queen Mary University of London
Chapters
- 00:05Title
- 01:06Zygote Isolation
- 03:31PN Injection
- 04:52Results: Evaluation of Gene Targeting in Golden Syrian Hamster by the Cas9/sgRNA System
- 05:36Conclusion
Pronuclear (PN) injection of the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein-9 nuclease (CRISPR/Cas9) system is a highly efficient method for producing genetically engineered golden Syrian hamsters. Herein, we describe the detailed PN injection protocol for the production of gene knockout hamsters with the CRISPR/Cas9 system.
