Zebrafish Husbandry
Zebrafish Husbandry - Related Videos
07:58
Zebrafish Maintenance and Husbandry
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2023
The zebrafish (Danio rerio) is a powerful vertebrate model system for studying development, modeling disease, and screening for novel therapeutics. Due to their small size, large numbers of zebrafish can be housed in the laboratory at low cost. Although zebrafish are relatively easy to maintain, special consideration must be given to both diet and water quality to in order to optimize fish health and reproductive success. This video will provide an overview of zebrafish husbandry and...
06:00
Forebrain Electrophysiological Recording in Larval Zebrafish
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Cited by 55 •
2013
A simple method to record extracellular field potentials in the larval zebrafish forebrain is described. The method provides a robust in vivo read-out of seizure-like activity. This technique can be used with genetically modified zebrafish larvae carrying epilepsy-related genes or seizures evoked by administration of convulsant drugs.
06:55
In vivo Electroporation of Morpholinos into the Adult Zebrafish Retina
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Cited by 42 •
2011
A method to conditionally knockdown a target protein’s expression in the adult zebrafish retina is described, which involves intravitreally injecting antisense morpholinos and electroporating them into the retina. The resulting protein is knocked down for several days, which allows testing the protein’s role in the regenerating or intact retina.
05:21
Training Laboratory Rabbits to Refine Routine Husbandry Procedures
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Cited by 2 •
2024
Training protocols involving positive reinforcement for routine husbandry procedures in rabbits were developed and proved to be successful, as the rabbits reliably showed the trained behaviors at the weekly general examination. Picking the rabbits up with the transport box was less aversive than the conventional handling technique.
07:58
Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury
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Cited by 8 •
2016
Renal injuries incurred from nephrotoxins, which include drugs ranging from antibiotics to chemotherapeutics, can result in complex disorders whose pathogenesis remains incompletely understood. This protocol demonstrates how zebrafish can be used for disease modeling of these conditions, which can be applied to the identification of renoprotective measures.
FAQs
Temperature, pH, dissolved oxygen, filtration, and overall water quality work together to influence normal development, fertility, behavior, and survival. Because these conditions affect the animals across their life cycle, consistent monitoring and management are essential. Maintaining suitable values also helps reduce variation between tanks and supports more reproducible biological experiments.
Stocking density and light cycles are important environmental conditions within zebrafish husbandry. Density must be managed as part of maintaining suitable care conditions, while consistent light cycles help support normal biological function and behavior. Controlling both variables contributes to stable colony performance and reduces environmental differences that could influence developmental, behavioral, or physiological studies.
Filtration helps maintain the water conditions required for healthy animals, while disease prevention protects colony health and continuity. Together with tank cleaning and routine management, these measures support survival and normal development. Their importance extends beyond individual fish because unhealthy or inconsistent colonies can compromise fertility, behavior, and the reliability of results collected across experiments.
A basic workflow coordinates feeding, tank cleaning, water-quality management, filtration, and disease-prevention practices. Breeding activities are also organized when researchers need to maintain or expand the colony. Performing these tasks consistently supports suitable environmental conditions and helps preserve healthy animals for experiments involving development, genetics, physiology, disease mechanisms, drug responses, or environmental effects.
Careful colony management supports fertility and enables breeding across generations. Researchers coordinate breeding with feeding, tank care, water-quality control, and disease prevention so that animals remain healthy and reproductive performance is sustained. This continuity is important when biological studies depend on normal development, consistent traits, or the availability of zebrafish over multiple generations.
Biologists use well-managed colonies for studies of embryology, genetics, physiology, disease mechanisms, drug responses, and environmental effects. Husbandry is especially relevant when experimental outcomes depend on normal development, fertility, behavior, or survival. Consistent care provides a stable biological foundation, helping researchers distinguish effects associated with their study from variation caused by colony conditions.