Cho Cell Culture
Cho Cell Culture - Related Videos
07:06
Primary Clarification of CHO Harvested Cell Culture Fluid using an Acoustic Separator
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Cited by 7 •
2020
Presented here is a protocol for the primary clarification of CHO cell culture using an acoustic separator. This protocol can be used for the primary clarification of shake flask cultures or bioreactor harvests and has the potential application for continuous clarification of the cell bleed material during perfusion bioreactor operations.
08:15
Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells
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Cited by 11 •
2018
A detailed protocol for the concurrent operation of 48 parallel cell cultures under varied conditions in a microbioreactor system is presented. Cell culture process, harvest and subsequent antibody titer analysis are described.
01:13
meta-Directing Deactivators: –NO2, –CN, –CHO, –CO2R, –COR, –CO2H
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2023
All meta-directing substituents are deactivating groups. These substituents withdraw electrons from the aromatic ring, making the ring less reactive toward electrophilic substitution. For example, the nitration of nitrobenzene is 100,000 times slower than that of benzene because of the deactivating effect of the nitro group. The first step in an electrophilic aromatic substitution is the addition of an electrophile to form a resonance-stabilized carbocation. The energy diagrams for the...
08:05
Culturing Human Pluripotent and Neural Stem Cells in an Enclosed Cell Culture System for Basic and Preclinical Research
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Cited by 3 •
2016
Here is a protocol to grow pluripotent stem cells (PSC) and neural stem cells (NSC) in an enclosed cell culture system that permits maximum sterility and reproducibility, replacing the traditional biosafety cabinet and incubator. This equipment meets clinical good manufacturing practice (cGMP) and clinical good lab practice (cGLP) guidelines.
07:01
Induction and Testing of Hypoxia in Cell Culture
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Cited by 247 •
2011
Here we propose simple methods to induce hypoxia in cell cultures and simple tests to evaluate the hypoxic status of the cultures.
FAQs
Adherent and suspension growth provide different ways to maintain and expand CHO populations. Adherent cells grow attached to a surface, whereas suspension cells grow throughout the medium, making the culture format a central handling decision. Passaging transfers a portion of a healthy culture into fresh conditions, helping sustain continued expansion for experiments or production.
Temperature, carbon dioxide, osmolarity, and pH must remain controlled because CHO cell health and growth depend on a stable culture environment. Sterile nutrient medium supplies the chemical support for maintenance and expansion, while contamination control protects the population and the reliability of downstream measurements. Together, these variables influence whether cultures remain suitable for research or production.
CHO cells are valuable for recombinant protein work because they carry out mammalian protein processing. This capability helps generate complex biological molecules in forms relevant to research and biologic development. In cancer studies, that matters when investigators need antibodies or other proteins whose function depends on appropriate cellular processing.
Genetic engineering gives researchers a way to modify CHO cells for targeted protein production or functional studies. In cancer research, engineered cultures can support recombinant antibody and protein production, as well as experiments examining how a protein performs or how cells respond to a treatment. This makes the culture a platform for connecting molecular design with measurable biological outcomes.
A basic CHO cell culture workflow starts with sterile nutrient medium and a controlled environment. Researchers maintain cells under specified temperature, carbon dioxide, osmolarity, and pH conditions, then use either adherent or suspension growth. Passaging transfers healthy cells into fresh culture conditions to expand the population. The selected format and environmental controls determine how readily cultures can be sustained for assays or biologic production.
CHO cultures can support assays that measure protein function, cellular toxicity, and drug-related effects. These readouts address different questions: protein-function assays examine biological activity, toxicity assays evaluate harmful cellular responses, and drug-related studies assess effects associated with treatment. Using the same mammalian culture platform for these endpoints connects experimental manipulation with observable cellular or molecular outcomes.
The large-scale adaptability of CHO cultures supports work beyond an individual laboratory assay. Researchers can use these cultures to help develop and manufacture biologics, including products relevant to cancer diagnosis and treatment. Their mammalian processing capability is especially useful when complex proteins or recombinant antibodies are needed. This connects cell-culture research with translational development and production of cancer-related biologics.