Genetically Modified Keratinocytes
Genetically Modified Keratinocytes - Related Videos
04:07
Genetic Modification of Primary Human Keratinocytes: A Method for Genetically Manipulating Keratinocytes Using Recombinant Retroviruses
0 Views •
2023
This video describes a detailed protocol for producing genetically modified primary human keratinocytes recombinant retrovirus. This method can be used to manipulate gene expression in keratinocytes.
12:45
Testing For Genetically Modified Foods
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2023
Source: Laboratories of Margaret Workman and Kimberly Frye - Depaul University Genetic modification of foods has been a controversial issue due to debated concerns over health and environmental safety. This experiment demonstrates technical understanding of how food DNA is genetically identified, allowing for educated decision making about the safety and potential dangers of using genetically modified organisms (GMOs) in food supplies. Polymerase Chain Reaction (PCR) is used to amplify food DNA...
09:16
Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis
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Cited by 22 •
2015
The goal of this paper is to provide a comprehensive and detailed protocol on how to generate genetically modified human organotypic skin from epidermal keratinocytes and devitalized human dermis.
17:50
Preventing the Spread of Malaria and Dengue Fever Using Genetically Modified Mosquitoes
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Cited by 10 •
2007
In this candid interview, Anthony A. James explains how mosquito genetics can be exploited to control malaria and dengue transmission. Population replacement strategy, the idea that transgenic mosquitoes can be released into the wild to control disease transmission, is introduced as well as the concept of genetic drive and the design criterion for an effective genetic drive system. The ethical considerations of releasing genetically-modified organisms into the wild are also discussed.
06:44
Keratinocyte Colony Formation Assay: An In Vitro Assay to Assess the Ability of Keratinocytes to Grow into Colonies
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2023
In this video, we perform a clonogenic assay of keratinocytes harvested from adult mice to determine the ability of individual cells to proliferate and form colonies. This keratinocyte harvesting technique is useful for studying other downstream aspects of cutaneous chemical carcinogenesis and tumor promotion.
FAQs
An introduced genetic change can shift signaling networks that regulate proliferation, differentiation, or responses to treatment. Researchers compare the resulting cell behavior under controlled culture conditions to determine how the altered gene expression or trait influences epidermal biology. This makes it possible to connect a defined molecular change with measurable cellular consequences relevant to skin cancer.
Viral gene delivery and genome editing provide different routes for creating altered keratinocyte models. The first uses a viral system to deliver genetic material, while the second changes the cell’s genome through an editing approach. Together, these methods allow researchers to establish models carrying defined genetic alterations for investigating specific biological or cancer-related questions.
After modification, researchers can examine changes in signaling, cell proliferation, differentiation, and treatment response. These readouts are complementary: signaling changes indicate pathway effects, proliferation reflects growth control, differentiation reveals epidermal behavior, and treatment responses show how cells react under experimental conditions. Evaluating several outcomes provides a broader picture of the resulting cellular phenotype.
In cancer research, altered keratinocytes can represent oncogenic changes and help investigators examine pathways associated with tumor initiation and progression. Their value lies in linking a specific molecular alteration to cell-level behavior, then relating those findings to tissue-level phenotypes in skin cancer. This supports mechanistic studies of how genetic changes influence disease-relevant epidermal biology.
A basic workflow begins by introducing the selected genetic alteration through viral gene delivery or genome editing, followed by culturing the modified keratinocytes under controlled conditions. Researchers then assess signaling, proliferation, differentiation, or treatment responses. Maintaining controlled culture conditions helps investigators associate observed behavioral differences with the engineered change and evaluate its biological consequences.
Researchers use these models when they need to examine the consequences of a defined oncogenic change or altered pathway in epidermal cells. They can investigate how that change contributes to tumor initiation or progression, study skin biology, and test cellular responses to candidate treatments. The approach connects molecular events with observable cell-level and tissue-level effects.
Treatment experiments can show whether a genetic alteration changes how keratinocytes respond to a candidate therapy. By relating treatment-associated behavior to the underlying signaling, proliferation, or differentiation state, researchers can connect molecular context with experimental outcome. These findings support evaluation of potential therapies and help clarify whether a treatment effect is associated with the engineered cancer-relevant change.