Orthotopic Intracranial Gbm
Orthotopic Intracranial Gbm - Related Videos
05:10
Intracranial Orthotopic Allografting of Medulloblastoma Cells in Immunocompromised Mice
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Cited by 5 •
2010
This protocol describes the isolation and dissociation of mouse medulloblastoma tissue, and subsequent allografting of the tumor cells into immunocompromised recipient mice in order to initiate secondary medulloblastoma.
02:58
Intracranial Orthotopic Model: A Procedure to Implant Cancer Cells in Mouse Brain
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2023
This video demonstrates implantation of tumor cells in the mouse brain by performing intracranial injection.
03:02
Orthotopic Inoculation of Thyroid Cancer Cells into Murine Thyroid: A Procedure to Establish Orthotopic Thyroid Cancer Mouse Model
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2023
This video describes the procedure for orthotopic inoculation of thyroid cancer cells into the thyroid gland of mouse models, which may then serve as a valuable platform to assess carcinogenesis and discover potential treatment strategies.
01:55
Intracranial Injection of Gut-Derived Microbial Metabolites in a Mouse Model
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2025
Source: Liou, C. et al. Intracerebroventricular Delivery of Gut-Derived Microbial Metabolites in Freely Moving Mice. J. Vis. Exp. (2022).This video demonstrates the intracranial injection of short-chain fatty acids (SCFAs) in a freely moving mouse to study their effects on brain-mediated behavior. An injector cannula is inserted into a guide cannula implanted in the mouse brain. After recovery, the mouse acclimates in a novel cage. SCFAs are then administered, and locomotor behavior is recorded.
09:16
Orthotopic Small Bowel Transplantation in Rats
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Cited by 4 •
2012
Small bowel transplantation has become an accepted treatment option for patients with irreversible intestinal failure. Our experimental model of orthotopic small bowel transplantation in rats serves as a reliable tool to address underlying immunologic and inflammatory processes that complicate intestinal transplantation.
FAQs
Brain placement allows the developing tumor to remain in contact with neural tissue and brain-associated microenvironments. Those local interactions can influence how the tumor invades nearby tissue, progresses over time, and responds to treatment. Consequently, observations may reflect central nervous system conditions more closely than results from models established outside the brain.
This model supports analysis of local invasion, tumor progression, and treatment response within the brain environment. Researchers can therefore study glioblastoma behavior together with the surrounding neural tissue and associated microenvironments, rather than examining tumor growth independently of its anatomical setting. These observations contribute to a more context-specific understanding of disease biology.
The key distinction is anatomical context. An intracranial model places glioblastoma cells or tissue where they interact with neural tissue and brain-associated microenvironments, whereas models outside the brain do not reproduce that setting. This difference can make intracranial findings more representative of tumor growth and therapeutic response in the central nervous system.
They provide the local context in which tumor growth, invasion, and progression occur. Because the implanted tumor develops alongside neural tissue and other brain-associated features, investigators can evaluate treatment response under conditions that better represent the central nervous system. This context strengthens interpretation of how experimental therapies may perform against tumors in the brain.
The workflow begins by introducing glioblastoma cells or tumor tissue into the brain. The implanted material is then allowed to develop within the intracranial environment so researchers can examine tumor growth, local invasion, progression, or treatment response. The model therefore links tumor establishment with later biological and therapeutic assessments in the brain.
Researchers would select this approach when the study requires glioblastoma to be evaluated in its native anatomical environment. It is particularly relevant for investigating tumor biology, assessing anticancer therapies, and examining responses shaped by the central nervous system. Its use can improve the translational relevance of laboratory findings compared with studies conducted outside the brain.
Experiments can generate information about glioblastoma invasion, progression, and response to anticancer treatment within brain tissue. These findings may help investigators compare therapeutic effects under central nervous system conditions, identify how local context influences tumor behavior, and refine therapeutic strategies. The broader value lies in improving the translational relevance of preclinical cancer studies.