Nmr Active Nuclei
Nmr Active Nuclei - Related Videos
10:08
Nuclear Magnetic Resonance (NMR) Spectroscopy
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2023
Source: Laboratory of Dr. Henrik Sundén – Chalmers University of Technology Nuclear magnetic resonance (NMR) spectroscopy is a vital analysis technique for organic chemists. With the help of NMR, the work in the organic lab has been facilitated tremendously. Not only can it provide information about the structure of a molecule but also determine the content and purity of a sample. Compared with other commonly encountered techniques for organic chemists — such as thermal analysis and mass...
03:14
Isolating Cell Populations from Brain Tissue Using Fluorescence-Activated Nuclei Sorting
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2025
This video demonstrates the procedure for isolating and sorting nuclei from fresh-frozen human brain tissue using fluorescence-activated nuclei sorting (FANS), which enables the study of different cell populations for various neurological applications.
09:24
Application and Methodology of the Non-destructive 19F Time-domain NMR Technique to Measure the Content in Fluorine-containing Drug Products
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Cited by 2 •
2017
A simple and non-destructive technique that measures the average content of drug substances in formulated drug products containing fluorine using low-field fluorine-19 (19F) time-domain (TD) nuclear magnetic resonance (NMR) is presented here. The technique can be applied to the development and manufacturing of drugs in the pharmaceutical industry.
04:33
Quantitative In-Cell Hydrogen NMR Spectroscopy to Monitor Protein-Ligand Interactions
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2025
In this video, we demonstrate the in-cell nuclear magnetic resonance spectroscopy technique to study protein-ligand interactions between unlabeled overexpressed proteins and small molecules. The successful ligand-protein interaction is confirmed visually by the appearance of an additional set of peaks in the spectral region of interest that gradually replaces the original peaks.
16:20
Hyperpolarized Xenon for NMR and MRI Applications
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Cited by 28 •
2012
The production of hyperpolarized xenon by means of spin exchange optical pumping (SEOP) is described. This method yields a ~10000-fold enhancement of the nuclear spin polarization of Xe-129 and has applications in nuclear magnetic resonance spectroscopy and imaging. Examples of gas phase and solution state experiments are given.
FAQs
Nonzero nuclear spin gives a nucleus an associated magnetic moment, allowing it to respond to a strong external magnetic field. The field separates the allowed nuclear energy levels, and radiofrequency energy can drive transitions when its frequency matches the resonance condition. That response provides the measurable basis for comparing nuclei in a chemical sample.
Shielding and spin-spin coupling shape the spectrum in different ways. Shielding changes the magnetic environment experienced by a nucleus, so resonance positions can reflect differences in molecular surroundings. Spin-spin coupling records interactions between nuclei and can produce related signal patterns. Considering both effects helps chemists connect observed signals with bonding and molecular structure rather than treating each signal as an isolated peak.
Isotopic abundance is a major practical factor when comparing NMR-active nuclei. Even when a nucleus has the required spin and magnetic moment, its scarcity in the sample can reduce detection sensitivity relative to a more abundant isotope. Chemists therefore consider both whether a nucleus can generate an NMR response and how readily its signals can be observed.
NMR-active nuclei can report several levels of chemical information from the same sample. Signal behavior can support conclusions about molecular structure and bonding, while changes associated with conformation and dynamics reveal how molecules adopt states or move. This broader information makes the approach useful when composition alone is insufficient to distinguish compounds or molecular forms.
A chemistry workflow begins by selecting a nucleus whose NMR response is relevant to the question, then measuring its radiofrequency response in a strong external magnetic field. The resulting signals are examined using shielding and spin-spin coupling information. Interpreting those features can connect the measurement to structure, bonding, conformation, dynamics, or composition.
Because their signals reflect molecular environments, NMR-active nuclei can help follow changes during a chemical reaction and assess whether a sample contains the expected material. The same measurements support compound identification and composition analysis. In chemistry, these applications turn spectral observations into evidence about reaction progress, product identity, and sample quality.