Room Temperature Stirring

Room temperature stirring is the controlled agitation of a chemical mixture at approximately ambient temperature, promoting uniform composition without deliberate heating or cooling. A stir bar, magnetic stirrer, or mechanical impeller transfers motion through the solution, improving contact between reactants, dispersing dissolved or suspended materials, and supporting consistent mass and heat distribution. In chemistry, this method is widely used during reactions, dissolutions, extractions, crystallizations, and sample preparation when temperature-sensitive compounds or mild reaction conditions are important. Maintaining room temperature while stirring can improve reproducibility, limit heat-driven side reactions, and provide a practical baseline for comparing reaction behavior across experiments.

Room Temperature Stirring - Related Videos

Research

JoVE Journal - Bioengineering

Mammalian Cell Encapsulation in Alginate Beads Using a Simple Stirred Vessel

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Cited by 13 •

2017

This video and manuscript describe an emulsion-based method to encapsulate mammalian cells in 0.5% to 10% alginate beads which can be produced in large batches using a simple stirred vessel. The encapsulated cells can be cultured in vitro or transplanted for cellular therapy applications.

Research

JoVE Journal - Bioengineering
Free Sample

Power Input Measurements in Stirred Bioreactors at Laboratory Scale

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Cited by 9 •

2018

The power input in stirred bioreactors can be measured through the torque that acts on the impeller shaft during rotation. This manuscript describes how an air bearing can be used to effectively reduce friction losses observed in mechanical seals and improve the accuracy of power input measurements in small-scale vessels.

Education

JoVE Lab Manual - Chemistry

Temperature Dependence - Instructor Prep

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2020

Source: Smaa Koraym at Johns Hopkins University, MD, USA Preparation of 0.5 M Fe(NO3)3Expand Here, we show the preparation of a solution for 10 students working in pairs, with some excess. Please adjust quantities as needed. To set up for this lab experiment, wear the appropriate personal protective equipment, including a lab coat, chemical splash goggles, and gloves Prepare 250 mL of a 0.5 M aqueous solution of iron (III) nitrate. Measure out 50.5 g of iron nitrate...

Conducting Reactions Below Room Temperature

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2023

Source: Laboratory of Dr. Dana Lashley - College of William and Mary Demonstration by: Matt Smith When new bonds are formed in the course of a chemical reaction, it requires that the involved species (atoms or molecules) come in very close proximity and collide into one another. The collisions between these species are more frequent and effective the higher the speed at which these molecules are moving. A widely used rule of thumb, which has its roots in the Arrhenius equation1, states that...

Continuously-stirred Anaerobic Digester to Convert Organic Wastes into Biogas: System Setup and Basic Operation

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Cited by 34 •

2012

Laboratory-scale anaerobic digesters allow scientists to research new ways of optimizing existing applications of anaerobic biotechnology and to evaluate the methane producing potential of various organic wastes. This article introduces a generalized model for the construction, inoculation, operation, and monitoring of a laboratory-scale continuously stirred anaerobic digester.

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