Cd133 Sox9 Egfp
Cd133 Sox9 Egfp - Related Videos
05:40
Melanoma Stem Cell Isolation: A Method to Obtain CD133 Positive and Negative Melanoma Cells Using Magnetic-activated Cell Sorting
0 Views •
2023
This video describes the technique of isolating CD133 negative and positive melanoma stem cells from a primary melanoma cell line using magnetic-activated cell sorting. The CD133 positive melanoma CSCs are further studied to understand the mechanisms of melanoma tumorigenesis.
12:06
Isolation of CD133+ Liver Stem Cells for Clonal Expansion
0 Views •
Cited by 22 •
2011
Here we describe the isolation of CD133 expressing liver stem cells and cancer stem cells from whole murine liver, a process that requires tissue digestion, cell enrichment, and flow cytometry isolation. We include methods for advanced single cell isolation and clonal expansion.
11:43
Easy Measurement of Diffusion Coefficients of EGFP-tagged Plasma Membrane Proteins Using k-Space Image Correlation Spectroscopy
0 Views •
Cited by 37 •
2014
This paper provides a step by step guide to the fluctuation analysis technique k-Space Image Correlation Spectroscopy (kICS) for measuring diffusion coefficients of fluorescently labeled plasma membrane proteins in live mammalian cells.
10:03
Development of Leishmania Species Strains with Constitutive Expression of eGFP
0 Views •
2023
Here, we describe the methodology used for generating L. panamensis and L. donovani strains expressing the gene for eGFP as a stable integrated transgene using the pLEXSY system. Transfected parasites were cloned by limiting dilution, and clones with the highest fluorescence intensity in both species were selected for further use in drug screening assays.
10:20
Studying Membrane Protein Trafficking in Drosophila Photoreceptor Cells Using eGFP-Tagged Proteins
0 Views •
Cited by 1 •
2022
Here, non-invasive methods are described for localization of photoreceptor membrane proteins and assessment of retinal degeneration in the Drosophila compound eye using eGFP fluorescence.
FAQs
EGFP provides a visible readout of Sox9 regulatory activity, allowing researchers to compare where and when Sox9-associated programs are active. Changes in fluorescence can therefore be examined across developing tissues or developmental stages. This helps connect transcriptional regulation with the location and behavior of selected cell populations during tissue formation and regeneration.
CD133 adds a cell-surface labeling dimension to the Sox9-linked fluorescent signal. Its presence helps distinguish cells with associated progenitor or stem-like characteristics from other EGFP-marked cells. Considering both signals supports more focused interpretation of population identity, particularly when researchers examine whether Sox9 activity occurs within a specific developmental cell compartment.
The two markers provide complementary information: CD133 helps characterize a cell population, whereas EGFP indicates Sox9 regulatory activity. Their combination can reveal whether Sox9-associated activity is concentrated in CD133-associated cells or distributed more broadly. This comparison helps researchers investigate cellular heterogeneity and relate molecular activity to developmental population structure.
By identifying selected cells and tracking their presence across developing tissues, the system supports analysis of how those populations are associated with tissue formation, maintenance, and regeneration. Spatial examination adds information about where cells occur, while repeated assessment of marker patterns helps researchers investigate changes in developmental programs and possible lineage contributions.
A study can begin by identifying EGFP-positive cells and assessing CD133 labeling within the relevant developing tissue. Researchers can then visualize the marked population, isolate selected cells when needed, and compare their distribution or marker status across developmental conditions. The resulting observations can be used to evaluate Sox9 activity and population-associated developmental behavior.
Isolation is useful when investigators need to examine the selected population separately from surrounding tissue. CD133 labeling and Sox9-linked EGFP help guide identification of cells for focused analysis, while tissue imaging preserves information about their original location. Together, these approaches support comparisons between population characteristics, spatial distribution, and developmental state.
The system can be used to assess how marked populations relate to tissue formation, maintenance, and regeneration. Researchers may compare Sox9 activity and cell distribution during these processes, then examine whether associated populations change in abundance or location. Such observations provide a way to connect developmental programs with the organization and persistence of specific cells.
Development depends not only on which cells are present, but also on where they are located within a tissue. Fluorescent identification makes it possible to examine the distribution of Sox9-associated, CD133-labeled populations in developing structures. This spatial context can clarify how selected cells are positioned relative to tissue formation, maintenance, and regenerative processes.