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Easy Measurement of Diffusion Coefficients of EGFP-tagged Plasma Membrane Proteins Using k-Space Image Correlation Spectroscopy
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JoVE Journal Biology
Easy Measurement of Diffusion Coefficients of EGFP-tagged Plasma Membrane Proteins Using k-Space Image Correlation Spectroscopy

Easy Measurement of Diffusion Coefficients of EGFP-tagged Plasma Membrane Proteins Using k-Space Image Correlation Spectroscopy

DOI:
10.3791/51074-v

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11:43 min

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May 10, 2014

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, , , ,
1Institute of Molecular Biology and Genetics and Interdisciplinary Nanoscience Center,Aarhus University, 2Departments of Chemistry and Physics,McGill University

Chapters

  • 00:05Title
  • 01:15Import the Image Sequence of Interest into an Imaging Analysis Program
  • 02:37Perform the Analysis
  • 06:52Calculate Average of the Diffusion Coefficient
  • 07:56Results: Example ROI Crops, Diffusion, and k2 Plots
  • 11:09Conclusion

Summary

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Automatic Translation

This paper provides a step by step guide to the fluctuation analysis technique k-Space Image Correlation Spectroscopy (kICS) for measuring diffusion coefficients of fluorescently labeled plasma membrane proteins in live mammalian cells.

Tags

Diffusion CoefficientsEGFPPlasma Membrane ProteinsK-Space Image Correlation Spectroscopy (kICS)Fluorescence MicroscopyImage AnalysisAquaporin-3Membrane Protein Diffusion

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