Gland Formation Ability
Gland Formation Ability - Related Videos
06:44
Keratinocyte Colony Formation Assay: An In Vitro Assay to Assess the Ability of Keratinocytes to Grow into Colonies
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2023
In this video, we perform a clonogenic assay of keratinocytes harvested from adult mice to determine the ability of individual cells to proliferate and form colonies. This keratinocyte harvesting technique is useful for studying other downstream aspects of cutaneous chemical carcinogenesis and tumor promotion.
09:59
Transfer of Mammary Gland-forming Ability Between Mammary Basal Epithelial Cells and Mammary Luminal Cells via Extracellular Vesicles/Exosomes
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Cited by 8 •
2017
This protocol describes methods for purifying, quantitating, and characterizing extracellular vesicles (EVs)/exosomes from non-adherent/mesenchymal mammary epithelial cells and for using them to transfer mammary gland-forming ability to luminal mammary epithelial cells. EVs/exosomes derived from stem-like mammary epithelial cells can transfer this cell property to cells that ingest the EVs/exosomes.
03:06
The Effect of Aspirin and Ibuprofen on the Phagocytic Ability of Macrophages
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2025
Source:Adepemi O. Ogundeji1, Nozethu Mjokane1, Maphori Maliehe1, Olihile M. Sebolai11Department of Microbiology and Biochemistry, University of the Free State.This video showcases a method to assess the impact of aspirin and ibuprofen, at their minimum inhibitory concentrations, on macrophage phagocytosis using pH-sensitive fluorescent dye-stained fungal cells. The drugs inhibit cyclooxygenase-2, decreasing prostaglandin formation and boosting macrophage phagocytosis, as observed through...
05:13
Gastric Fundic Glands Isolation: A Method to Isolate Fundic Glands from Human Stomach Tissue
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2023
This video describes a method to isolate gastric epithelial cells from the human fundic tissue. The isolated epithelial cells can be used as an in vitro model to study their regulatory roles in gastric physiology and pathophysiology.
09:34
Assessing Differences in Sperm Competitive Ability in Drosophila
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Cited by 8 •
2013
Differential sperm competitive ability among Drosophila males with distinct genotypes can be ascertained through double-mating experiments. Each of these experiments involves one of the males of interest and a reference male. Readily identifiable markers in the progeny allow inference of the fraction of individuals fathered by each male.
FAQs
Epithelial cells must differentiate while communicating with surrounding mesenchymal cells, whose reciprocal signals help guide tissue organization. This coordination links cellular specialization with physical changes such as invagination, budding, and branching. Studying these interactions helps explain how developing tissue progresses from an organized epithelial structure toward a gland with defined architecture and functional potential.
Invagination and budding reshape an epithelial sheet or tissue, while branching expands its developing architecture. These processes are coordinated rather than isolated events, because tissue geometry must support lumen formation and later functional maturation. Examining their sequence allows developmental biologists to connect cellular behavior with the emergence of the complex structures characteristic of endocrine and exocrine glands.
Lumen formation provides an architectural step between early tissue remodeling and a mature glandular structure. Its coordination with epithelial differentiation and branching helps organize how cells are arranged within the developing organ. Consequently, lumen development serves as an important indicator when assessing whether a model reproduces gland organogenesis rather than merely producing differentiated cells.
Disrupting the reciprocal signals between epithelial and mesenchymal cells can interfere with differentiation, tissue remodeling, branching, lumen formation, or functional maturation. Because these events are coordinated, a disturbance at one stage may alter later architecture and performance. This relationship makes gland development a useful framework for investigating how abnormal developmental processes may contribute to congenital abnormalities.
Organoid systems can be used to recreate selected features of gland development in vitro, including epithelial differentiation, tissue organization, branching, lumen formation, and maturation. Researchers can examine whether these features emerge in a coordinated sequence and compare the resulting architecture with developmental expectations. Such models provide a controlled setting for studying mechanisms that are difficult to isolate in developing organs.
It shows how organogenesis depends on the integration of cell specialization, tissue remodeling, intercellular signaling, and structural organization. Focusing on gland development connects molecular or cellular events with the formation of endocrine and exocrine organs. This perspective helps researchers analyze how developmental signals establish organ architecture and how coordinated processes produce functional tissues.
Knowledge of gland development supports efforts to model tissue formation, examine developmental abnormalities, and investigate regeneration. Organoid systems can reproduce aspects of gland architecture and maturation, while disease models can explore how disrupted signaling changes those outcomes. These applications make developmental mechanisms relevant beyond embryology, particularly when studying how organized glandular tissue might be recreated or analyzed in vitro.