Human Amniotic Membrane
Human Amniotic Membrane - Related Videos
09:34
Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
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Cited by 3 •
2017
This protocol describes the reprogramming of primary amniotic fluid and membrane mesenchymal stem cells into induced pluripotent stem cells using a non-integrating episomal approach in fully chemically defined conditions. Procedures of extraction, culture, reprogramming, and characterization of the resulting induced pluripotent stem cells by stringent methods are detailed.
10:00
In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane
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Cited by 8 •
2019
This protocol describes the isolation of epithelial cells from different anatomical regions of the human amniotic membrane to determine their heterogeneity and functional properties for possible application in clinical and physiopathological models.
04:21
Lumican Extraction from Amniotic Membrane and Determination of its Storage Temperature
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2022
The present protocol describes the extraction of lumican from the amniotic membrane (AM) and their storing conditions as AM extract (AME) at -20 °C, 4 °C, and room temperature (RT) for 6, 12, 20, and 32 days to quantify its proteins and lumican concentration.
10:22
Enrichment of Bruch's Membrane from Human Donor Eyes
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Cited by 14 •
2015
Identifying proteins specifically associated with Bruch’s membrane in human eyes is an important step in understanding the biochemical mechanisms behind eye diseases such as age-related macular degeneration. This protocol describes how to enrich this sheet of extracellular matrix for down-stream biochemical analysis.
07:36
Modeling Encephalopathy of Prematurity Using Prenatal Hypoxia-ischemia with Intra-amniotic Lipopolysaccharide in Rats
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Cited by 20 •
2015
Encephalopathy of prematurity encompasses the central nervous system abnormalities associated with injury from preterm birth. This report describes a clinically relevant rat model of in utero transient systemic hypoxia-ischemia and intra-amniotic lipopolysaccharide administration (LPS) that mimics chorioamnionitis, and the related impact of infectious stimuli and placental underperfusion on CNS development.
FAQs
The epithelial layer and basement membrane work together rather than serving as an inert covering. The epithelium contributes to surface integrity, while the basement membrane and underlying matrix help establish a selective interface with amniotic fluid. Their organization creates a local signaling environment that can influence how developing tissues are protected and how epithelial behavior is maintained.
Because the connective tissue is avascular, the membrane provides structural and signaling functions without being organized around its own blood-vessel network. Its extracellular matrix, growth factors, and cell populations can affect epithelial integrity, inflammation, and tissue repair. Studying these components helps connect membrane structure with developmental protection and regenerative behavior.
Inflammation and repair are linked outcomes to examine when studying this tissue. Signals associated with the extracellular matrix and growth factors can influence the local response, while epithelial integrity reflects how well the interface remains maintained. Considering both outcomes prevents analysis from treating the membrane only as a physical barrier and supports a fuller view of its biological activity.
Preserved membrane serves as a research material for wound-healing studies, biomaterial development, and tissue-engineering strategies. Investigators can use it to examine how its matrix, growth factors, and cellular features relate to epithelial repair and inflammatory behavior. This translational use connects properties observed in fetal membranes with efforts to design or evaluate regenerative approaches.
Within developmental biology, the membrane offers a way to study how fetal surroundings are maintained during placental and fetal development. Its interface with amniotic fluid, selective barrier properties, and signaling components provide context for examining fetal protection. Research can therefore relate membrane organization to epithelial integrity, local signaling, and the broader biology of the developing conceptus.
Useful outcomes include the condition of epithelial integrity, the behavior of inflammatory responses, and indicators of tissue repair. These measures reflect different contributions of the membrane: epithelial organization concerns barrier maintenance, inflammatory behavior reflects signaling effects, and repair shows regenerative relevance. Examining them together can clarify why preserved membrane is valuable beyond its structural role.