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10:22 min
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November 15, 2015
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Summary
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Identifying proteins specifically associated with Bruch’s membrane in human eyes is an important step in understanding the biochemical mechanisms behind eye diseases such as age-related macular degeneration. This protocol describes how to enrich this sheet of extracellular matrix for down-stream biochemical analysis.
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Cite this Article
McHarg, S., Brace, N., Bishop, P. N., Clark, S. J. Enrichment of Bruch's Membrane from Human Donor Eyes. J. Vis. Exp. (105), e53382, doi:10.3791/53382 (2015).
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