Patient Tissue Samples
Patient Tissue Samples - Related Videos
08:40
Methyl-binding DNA capture Sequencing for Patient Tissues
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Cited by 1 •
2016
Here we present a protocol to investigate genome wide DNA methylation in large scale clinical patient screening studies using the Methyl-Binding DNA Capture sequencing (MBDCap-seq or MBD-seq) technology and the subsequent bioinformatics analysis pipeline.
07:32
Rapid Isolation of Viable Circulating Tumor Cells from Patient Blood Samples
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Cited by 36 •
2012
Circulating tumor cells are isolated from the blood of cancer patients without inflicting cellular damage. Isolation of tumor cells is accomplished using a bimolecular surface of E-selectin in addition to antibodies against epithelial markers. A nanotube coating specifically promotes cancer cell adhesion resulting in high capture purities.
08:48
Quantitation of γH2AX Foci in Tissue Samples
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Cited by 7 •
2010
Quantitation of DNA double-strand breaks on the basis of γH2AX foci has become an invaluable tool, particularly in radiation biology, for the evaluation of tissue radiosensitivity and effects of radiation modifying compounds. Here we demonstrate the use of an immunofluorescence assay for quantitation of γH2AX foci in tissue samples.
02:08
A Hydrophobic Tissue Clearing Method for a Rat Brain Tissue Sample
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2025
Source: Kirchner, K.N., et al., A Hydrophobic Tissue Clearing Method for Rat Brain Tissue. J. Vis. Exp. (2020).This video demonstrates the method of hydrophobic tissue clearing of a rat brain tissue sample. The sample is sequentially dehydrated in methanol, treated with a dichloromethane and methanol mixture to dissolve lipids and pigments, and transferred to dibenzyl ether for complete tissue clearing, enabling detailed imaging without thin sectioning.
05:00
Ex Vivo Culture of Patient Tissue & Examination of Gene Delivery
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Cited by 4 •
2010
This article describes the culture of patient tissue slices for gene delivery studies and subsequent analysis of gene expression using IVIS bioluminescence imaging.
FAQs
Preserving tissue integrity is essential because downstream genetic tests depend on usable nucleic acids. After collection, the sample can be processed to extract DNA or RNA. DNA supports sequencing and genotyping, whereas RNA enables gene-expression analysis. The quality of preservation therefore influences which molecular questions the specimen can answer and whether its findings can be interpreted reliably.
The distinction helps researchers interpret whether a finding reflects inherited variation or a somatic mutation associated with disease. Patient tissue samples can support separate investigative goals, including identifying inherited variants, detecting mutations in cancer, and recognizing molecular patterns linked to other conditions. This separation contributes to disease classification and to research on treatment strategies.
Clinical information gives genetic findings a medical context, helping researchers relate molecular patterns to health or disease. Consent establishes appropriate permission for collecting and using the specimen, while privacy protections help safeguard linked personal information. Together, these measures allow patient tissue samples to support research and genetic medicine while respecting the individual who provided them.
A typical workflow begins with collection through biopsy or surgery. The tissue is then preserved to maintain its integrity, processed in the laboratory, and used for DNA or RNA extraction. Researchers can apply sequencing, genotyping, or gene-expression analysis to the extracted material. The resulting molecular data may be considered alongside relevant clinical information.
DNA-based analysis is suited to examining genetic variation through approaches such as sequencing and genotyping. RNA-based analysis supports measurement of gene expression, providing information about molecular activity in the tissue. Using one or both types of extracted material lets researchers investigate different aspects of a specimen, from genetic variants to disease-associated expression patterns.
These specimens are valuable when researchers need molecular evidence connected to an individual’s health or disease. Applications include identifying inherited variants, studying somatic mutations in cancer, classifying disease, supporting diagnosis, and investigating treatments. When findings are combined with clinical information and appropriate protections, the samples also contribute to the development of more precise genetic medicine.