Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Quantitation of γH2AX Foci in Tissue Samples
JoVE Journal
Biology
Author Produced

A subscription to JoVE is required to view this content.

JoVE Journal Biology
Quantitation of γH2AX Foci in Tissue Samples
Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions
Journal (Biology)
Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions
Efficient and Consistent Generation of Retinal Pigment Epithelium/Choroid Flatmounts from Human Eyes for Histological Analysis
Journal (Biology)
Efficient and Consistent Generation of Retinal Pigment Epithelium/Choroid Flatmounts from Human Eyes for Histological Analysis
 

Quantitation of γH2AX Foci in Tissue Samples

Article DOI: 10.3791/2063 • 08:48 min • June 28th, 2010
June 28th, 2010 •
1,2, 1,3, 1,2,3, 1, 2,3, 3,4,5, 1,3
1Epigenomic Medicine, Baker IDI Heart and Diabetes Institute, The Alfred Medical Research and Education Precinct, 2Epigenetics in Human Health and Disease, Baker IDI Heart and Diabetes Institute, The Alfred Medical Research and Education Precinct, 3Department of Pathology, The University of Melbourne, 4Department of Allergy and Immunology, Murdoch Children's Research Institute, Royal Children's Hospital, 5Department of Pediatrics, The University of Melbourne

Chapters

Summary
Automatic Translation

Quantitation of DNA double-strand breaks on the basis of γH2AX foci has become an invaluable tool, particularly in radiation biology, for the evaluation of tissue radiosensitivity and effects of radiation modifying compounds. Here we demonstrate the use of an immunofluorescence assay for quantitation of γH2AX foci in tissue samples.

Tags

Quantitation γH2AX Foci Tissue Samples DNA Double-strand Breaks Endogenous Processes Exogenous Factors Ionizing Radiation Radiomimetic Compounds H2A Histone Variant Serine-139 Residue Phosphorylation PI3K Family Of Proteins ATM Kinase ATR Kinase Nuclear γH2AX Foci Immunofluorescence Microscopy Marker Specificity And Sensitivity Biomedical Research Radiation Biology Nuclear Medicine Cell Culture Systems Ionizing Radiation-induced DSBs Cellular Radiosensitivity Radiation-modifying Compounds DSB-inducing Compounds
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter