Allantoic Fluid Harvest
Allantoic Fluid Harvest - Related Videos
05:02
Purification of Virus from the Allantoic Fluid of Embryonated Chicken Eggs
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2026
Source: Yates, J. G. E., et al. Production of High-Titer Recombinant Newcastle Disease Virus from Allantoic Fluid. J. Vis. Exp. (2022)The video demonstrates the purification and concentration of virus particles from clarified allantoic fluid collected from virus-infected embryonated chicken eggs. It showcases a series of filtration and separation steps, including depth filtration, tangential flow filtration, and density gradient ultracentrifugation to isolate intact viruses. The virus-rich...
07:24
Production of High-Titer Recombinant Newcastle Disease Virus from Allantoic Fluid
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Cited by 3 •
2022
Here we provide a detailed procedure for production, purification, and quantification of high-titer recombinant Newcastle disease virus. This protocol consistently yields > 6 × 109 plaque-forming units/mL, providing virus quantities appropriate for in vivo animal studies. Additional quality control assays to ensure safety in vivo are described.
03:12
Propagation and Harvest of Influenza Virus from a Fertilized Chicken Egg
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2026
Source: Brauer, R. et al., Influenza Virus Propagation in Embryonated Chicken Eggs. J. Vis. Exp. (2015)This video demonstrates the synthesis and harvest of the influenza virus in fertilized chicken eggs. The virus is inoculated into the allantoic cavity, where it replicates in epithelial cells and buds into the allantoic fluid, which is then collected and stored for downstream applications.
04:55
Mesenteric Lymph Duct Cannulation in a Rat Model to Harvest Lymph Fluid
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2025
This video demonstrates a technique to cannulate the mesenteric lymph duct in rats. This method enables direct access to the entire volume of components present in the lymph fluid.
07:06
Primary Clarification of CHO Harvested Cell Culture Fluid using an Acoustic Separator
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Cited by 7 •
2020
Presented here is a protocol for the primary clarification of CHO cell culture using an acoustic separator. This protocol can be used for the primary clarification of shake flask cultures or bioreactor harvests and has the potential application for continuous clarification of the cell bleed material during perfusion bioreactor operations.
FAQs
Controlled incubation provides the conditions needed for an inoculated pathogen to replicate within the embryonated egg. As replication proceeds, infectious material accumulates in the allantoic fluid, creating a harvest suitable for later analysis. This relationship between incubation and accumulation is important because the collected fluid serves as the source material for infectivity measurements, immunological testing, or preparation of viral stocks.
The allantoic cavity provides an accessible environment in which infectious agents can replicate and accumulate in fluid that can later be collected. Influenza viruses are particularly associated with this application. By linking pathogen replication with a recoverable fluid sample, the system supports both host-pathogen studies and practical infection research without requiring analysis of the entire embryonated egg.
Clarification prepares the collected fluid for downstream evaluation after it has been withdrawn from the cavity. The clarified material can then be analyzed for infectivity or used in tests of antibody-mediated inhibition. It may also provide starting material for viral stocks or antigen production, making clarification an important transition between collection and immunological or virological applications.
The workflow begins by inoculating the pathogen into the allantoic cavity of an embryonated chicken egg. The egg is then incubated under controlled conditions to allow replication and accumulation in the fluid. After incubation, researchers open the cavity and withdraw the fluid aseptically, then clarify the harvest before measuring infectivity or using it in related applications.
Researchers can use clarified allantoic fluid as infectious material in experiments that measure whether antibodies inhibit pathogen activity. The harvest supplies a consistent sample for comparing infectivity in the presence or absence of antibody-mediated inhibition. This application connects pathogen propagation in the egg with immunological analysis of how antibodies affect infectious activity.
After pathogen replication and fluid collection, the harvest can support preparation of viral stocks or antigens for immunological assays and vaccines. Its value comes from concentrating infectious or antigenic material in a recoverable fluid phase. In infection research, this enables the same propagation system to support both experimental analysis and the preparation of materials for later studies.