Colloidal Gold Preparation
Colloidal Gold Preparation - Related Videos
08:21
Fabrication of Periodic Gold Nanocup Arrays Using Colloidal Lithography
0 Views •
Cited by 1 •
2017
We demonstrate the fabrication of periodic gold nanocup arrays using colloidal lithographic techniques and discuss the importance of nanoplasmonic films.
11:48
Development of a Colloidal Gold-based Immunochromatographic Test Strip for Detection of Cetacean Myoglobin
0 Views •
Cited by 5 •
2016
This protocol describes the development of two IgG class monoclonal antibodies (mAbs) strongly reactive to myoglobin of cetaceans. These mAbs are applied on a colloidal gold immunochromatographic test strip based on the sandwich format to differentiate the Mb of cetaceans from seal and other animals.
03:22
Colloids
0 Views •
2020
Children at play often make suspensions such as mixtures of mud and water, flour and water, or a suspension of solid pigments in water known as tempera paint. These suspensions are heterogeneous mixtures composed of relatively large particles that are visible to the naked eye or can be seen with a magnifying glass. They are cloudy, and the suspended particles settle out after mixing. On the other hand, a solution is a homogeneous mixture in which no settling occurs and in which the dissolved...
10:46
Preparation and Photoacoustic Analysis of Cellular Vehicles Containing Gold Nanorods
0 Views •
Cited by 5 •
2016
We show the preparation and address the feasibility of cellular vehicles containing gold nanorods for the photoacoustic imaging of cancer.
09:26
Synthesis and Characterization of Supramolecular Colloids
0 Views •
Cited by 3 •
2016
A protocol for the synthesis and characterization of colloids coated with supramolecular moieties is described. These supramolecular colloids undergo self-assembly upon the activation of the hydrogen-bonds between the surface-anchored molecules by UV-light.
FAQs
Particle size and dispersion depend on the chemical and physical conditions used during preparation. Changes in reagent concentrations, temperature, or mixing can alter how evenly the gold particles form and remain suspended. Controlling these variables is therefore important when the particles will later serve as detection labels, because dispersion affects the consistency of their biological use.
Citrate acts as a stabilizing agent that helps prevent gold particles from aggregating in solution. Maintaining separated particles supports a stable suspension and preserves the usable surface needed for later attachment of antibodies or other ligands. If dispersion is poorly controlled, the resulting material may be less suitable for biological detection formats.
Antibodies or other ligands attached to the particle surface provide molecular recognition of specific antigens. When a matching antigen is present, the functionalized particles bind to that target, linking gold’s optical properties with biological specificity. This combination allows researchers to detect pathogens, cellular structures, or immune responses in a targeted manner.
Preparation begins with chloroauric acid in solution, followed by chemical reduction to produce nanoscale gold particles. Stabilizing agents such as citrate are used to limit aggregation, while reagent concentrations, temperature, and mixing conditions are controlled to influence particle size and dispersion. The resulting suspension can then be functionalized for a selected detection application.
Immunogold electron microscopy is suited to high-resolution detection of pathogens or cellular structures, where the location of labeled targets can be examined at the microscopic level. Lateral-flow assays provide a rapid format for visible detection. Both rely on functionalized gold particles, but the choice depends on whether spatial resolution or rapid assay readout is the priority.
After particles are functionalized with antibodies or other binding ligands, they can be incorporated into immunogold electron microscopy, lateral-flow assays, and related diagnostic formats. These systems connect antigen recognition with either visible or high-resolution signals. Applications include detecting pathogens, examining cellular structures, and assessing immune responses in biological samples.