This protocol describes the development of two IgG class monoclonal antibodies (mAbs) strongly reactive to myoglobin of cetaceans. These mAbs are applied on a colloidal gold immunochromatographic test strip based on the sandwich format to differentiate the Mb of cetaceans from seal and other animals.
Chan, K., Lo, C., Chu, C., Chin, L., Wang, Y., Yang, W. Development of a Colloidal Gold-based Immunochromatographic Test Strip for Detection of Cetacean Myoglobin. J. Vis. Exp. (113), e53433, doi:10.3791/53433 (2016).