Dextran Peg Separation
Dextran Peg Separation - Related Videos
07:50
Electrophysiology of Scorpion Peg Sensilla
0 Views •
Cited by 4 •
2011
This article describes an electrophysiological method for isolating chemical stimulation to individual sensilla via extracellular, tip-recordings under mineral oil.
06:41
A Peg Plate Biofilm Assay for Screening Antibacterial Agents
0 Views •
2025
This video demonstrates an assay for screening antimicrobial compounds that efficiently eliminate biofilms using a peg-plate. The methodology monitors the biofilm's metabolic state after treatment with antimicrobial compounds, facilitating the determination of the minimum biofilm eradication concentration.
12:00
Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles
0 Views •
Cited by 9 •
2015
The objective of this protocol is to incorporate SDF-1α, a stem cell homing factor, into dextran sulfate-chitosan nanoparticles. The resultant particles are measured for their size and zeta potential, as well as the content, activity, and in vitro release rate of SDF-1α from the nanoparticles.
06:56
Particles without a Box: Brush-first Synthesis of Photodegradable PEG Star Polymers under Ambient Conditions
0 Views •
Cited by 17 •
2013
Poly(ethylene glycol) (PEG) brush-arm star polymers (BASPs) with narrow mass distributions and tunable nanoscopic sizes are synthesized in via ring opening metathesis polymerization (ROMP) of a PEG-norbornene macromonomer followed by transfer of portions of the resulting living brush initiator to vials containing varied amounts of a rigid, photo-cleavable bis-norbornene crosslinker.
02:56
FITC-Dextran Feeding: A Method to Quantify Intestinal Permeability in C. elegans
0 Views •
2023
This video introduces a method of to visualize and measure the integrity of the C. elegans intestine lumen by feeding worms FITC-labeled dextran. The example protocol shows the assay done with 3,3’-diindolylmethane (DIM)-treated and pathogen-fed worms.
FAQs
The polymers form separate aqueous phases when mixed at suitable concentrations because they partition differently in water. Cells then distribute between these phases rather than remaining uniformly suspended. Their location reflects combined effects of cell-surface properties, size, density, and interactions with each polymer, allowing researchers to favor one cellular population over others.
Partitioning depends on several cell characteristics acting together. Surface properties affect how cells interact with dextran and PEG, while size and density also influence their distribution. These differences can separate leukocytes, erythrocytes, and other sample components, although the outcome reflects the combined behavior of the cells and polymers rather than a single physical feature.
Polymer concentration is a central condition because it determines whether the mixed solution forms the aqueous two-phase system needed for separation. Once the phases develop, polymer interactions with cells help determine their distribution. Selecting suitable concentrations therefore supports enrichment or removal of targeted populations, while unsuitable conditions may not produce the intended partitioning pattern.
A typical workflow combines the biological sample with dextran and PEG at suitable concentrations, allowing the polymers to form separate aqueous phases. Cells then distribute according to their properties and polymer interactions. The phase containing the desired population can be collected or used for subsequent preparation, producing an enriched or depleted sample for downstream work.
The technique is useful when a complex biological sample contains cellular components that need to be enriched or removed before analysis or culture. In immunology, researchers may use its differing partition behavior to prepare leukocyte-containing fractions or reduce erythrocyte contributions. This preliminary separation can simplify later handling and support studies requiring relatively purified immune-cell populations.
Its gentle operation can help prepare viable cellular populations for downstream assays, while its scalability supports processing of complex samples. Infection researchers can use separated leukocyte, erythrocyte, or other fractions in pathogen-associated cell studies. Preparing the sample before analysis or culture may improve the suitability of the recovered populations for immunological investigation.