Ex Vivo Ctl Expansion
Ex Vivo Ctl Expansion - Related Videos
07:18
HLA-Ig Based Artificial Antigen Presenting Cells for Efficient ex vivo Expansion of Human CTL
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Cited by 17 •
2011
A new DC independent method for induction and expansion of antigen-specific T cells is described. HLA A2-Ig based artificial Antigen Presenting Cells (aAPC) are loaded with HLA-A2 restricted peptides to efficiently expand CTL of diverse antigen specificity. This technology holds great potential for CTL-based adoptive immunotherapy.
08:06
Experimental Metastasis and CTL Adoptive Transfer Immunotherapy Mouse Model
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Cited by 21 •
2010
An experimental lung metastasis and CTL immunotherapy mouse model for analysis of tumor cells-T cell interaction in vivo.
03:14
Ex Vivo Expansion of Natural Killer Cells from Peripheral Blood Mononuclear Cells
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2025
This video demonstrates a technique for the expansion of natural killer (NK) cells directly from peripheral blood mononuclear cells (PBMCs) using a feeder cell line. PBMCs are co-cultured with genetically modified lymphoblastoid feeder cells expressing membrane-bound interleukin-21 (mIL-21), and the culture is supplemented with the cytokines interleukin-2 (IL-2) and interleukin-15 (IL-15). Together, this cocktail of ligands leads to the prolonged expansion of NK cells with high purity.
07:20
Ex vivo Expansion of Tumor-reactive T Cells by Means of Bryostatin 1/Ionomycin and the Common Gamma Chain Cytokines Formulation
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Cited by 6 •
2011
An efficient protocol for the ex vivo expansion of tumor-reactive T cells from tumor-draining lymph nodes or other secondary lymphoid tissues of tumor-bearing hosts is described. This protocol selectively expands tumor-specific T cells for use in adoptive immunotherapy of breast cancer.
09:42
Analysis of Thermal Expansion via Dilatometry
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2023
Source: J. Jacob Chavez, Ryan T. Davis, and Taylor D. Sparks, Department of Materials Science and Engineering, The University of Utah, Salt Lake City, UT Thermal expansion is extremely important when considering which materials will be used in systems that experience fluctuations in temperature. A high or low thermal expansion in a material may or may not be desirable, depending on the application. For instance, in a common liquid thermometer, a material with a high thermal expansion would be...
FAQs
Expansion depends on coordinating two signal types: antigen-receptor or T-cell receptor stimulation directs the relevant T-cell population, while cytokines provide growth-supporting signals that promote proliferation. These inputs must be adjusted as a functional system, because maximizing cell number alone may compromise antigen specificity, viability, or cytotoxic activity. The appropriate balance determines experimental usefulness.
Cell proliferation does not by itself demonstrate that the expanded population remains useful. CTLs need to retain the capacity to recognize and destroy infected target cells, especially when the goal is to evaluate antiviral activity or support adoptive cell therapy. Therefore, yield should be considered alongside antigen specificity, viability, and functional cytotoxic activity.
Antigen-specific cells allow investigators to connect CTL activity with a defined immune target rather than treating proliferation as the sole outcome. In infection research, this distinction helps clarify cellular immune responses and supports assessment of whether expanded cells can recognize infected targets. Specificity is therefore central to interpreting antiviral function, not merely a feature of cell production.
An experimental workflow begins by isolating T cells, followed by stimulation of their antigen receptors or T-cell receptors. Growth-supporting cytokines are then supplied to promote proliferation outside the body. The resulting population is considered in terms of cell yield, antigen specificity, viability, and retained cytotoxic function, since these measures determine whether the preparation suits its intended use.
They can be used to clarify cellular immune responses, examine recognition and destruction of infected target cells, and evaluate antiviral activity. These applications make the cells useful for studying how immune defenses operate against infection and for comparing whether an expanded population retains the functional properties needed for a meaningful experimental readout.
Expansion supplies a larger population of CTLs for potential transfer-based treatment approaches, but cell number is not the only criterion. Conditions must preserve the desired antigen specificity, viability, and cytotoxic activity while promoting proliferation. This balance is important when expanded cells are being considered for adoptive cell therapy rather than used only as an experimental model.