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Ex vivo Expansion of Tumor-reactive T Cells by Means of Bryostatin 1/Ionomycin and the Common Gamma Chain Cytokines Formulation
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Immunology and Infection
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JoVE Journal Immunology and Infection
Ex vivo Expansion of Tumor-reactive T Cells by Means of Bryostatin 1/Ionomycin and the Common Gamma Chain Cytokines Formulation

Ex vivo Expansion of Tumor-reactive T Cells by Means of Bryostatin 1/Ionomycin and the Common Gamma Chain Cytokines Formulation

DOI:
10.3791/2381-v

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07:20 min

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January 14, 2011

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, , , ,
1Department of Microbiology & Immunology,Virginia Commonwealth University- Massey Cancer Center, 2Department of Internal Medicine,Virginia Commonwealth University- Massey Cancer Center, 3Department of Surgery,Virginia Commonwealth University- Massey Cancer Center

Chapters

  • 00:05Title
  • 00:55Introduction
  • 01:48Mouse Model of Breast Cancer
  • 02:14Isolation of Lymphocytes
  • 03:44Determine Fold Expansion of T Cells by Cell Counts and Flow Cytometry Analysis
  • 04:58Determine Tumor-Specificity of the ex vivo Expanded T Cells
  • 05:47Selective Expansion of Tumor-Reactive T Cells with Effector Anti-Tumor Function
  • 06:53Conclusion

Summary

Automatic Translation

Automatic Translation

An efficient protocol for the ex vivo expansion of tumor-reactive T cells from tumor-draining lymph nodes or other secondary lymphoid tissues of tumor-bearing hosts is described. This protocol selectively expands tumor-specific T cells for use in adoptive immunotherapy of breast cancer.

Tags

Ex Vivo ExpansionTumor-reactive T CellsBryostatin 1/IonomycinCommon Gamma Chain Cytokines FormulationBreast CancerImmune ResponsesAdoptive ImmunotherapyTumor-specific T CellsTumor AntigensIL-2IL-7IL-15IL-21FVBN202 Transgenic Mouse ModelHER-2/neu Positive Breast CarcinomaAdoptive T Cell Therapy

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