Infrared Dye Assay
Infrared Dye Assay - Related Videos
09:58
An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides
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Cited by 14 •
2016
We describe here an optimized protocol of fluorescent Electrophoretic Mobility Shift Assays (fEMSA) using purified SOX-2 proteins together with infrared fluorescent dye-labeled DNA probes as a case study to tackle an important biological question.
06:01
A Dye Release Assay to Quantify Enzymatic Activity of Antimicrobial Proteins
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2025
This video demonstrates the dye release assay for quantifying the enzymatic activity of antimicrobial proteins. The antimicrobial protein degrades the bacteria's dye-labeled cell wall and releases the dye molecules turning the solution blue. The absorbance of the solution correlates with the enzymatic activity of the antimicrobial protein.
06:13
Dyeing Insects for Behavioral Assays: the Mating Behavior of Anesthetized Drosophila
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Cited by 8 •
2015
This protocol describes a simple, cost effective way to individually identify Drosophila or other insects. Demonstration data investigating mating success across three species of Drosophila show that this method is comparable or better than the use of CO2 anaesthesia.
01:19
Assessing Bacterial Biofilm Formation Using a Cationic Dye-Based Assay
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2026
Begin with a bacterial suspension in nutrient media.Add the suspension to a multi-well plate and incubate.The nutrients in the media facilitate bacterial growth and attachment.The adhered bacteria secrete extracellular polymeric substances consisting of polysaccharides, proteins, and DNA to form a layer of biofilm.Wash multiple times with buffer to remove unbound bacteria.Invert the plate to ensure the removal of residual liquid from the well and allow the biofilm to dry.Treat with a fixative...
10:30
Dye-sensitized Solar Cells
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2023
Source: Tamara M. Powers, Department of Chemistry, Texas A&M University Today's modern world requires the use of a large amount of energy. While we harness energy from fossil fuels such as coal and oil, these sources are nonrenewable and thus the supply is limited. To maintain our global lifestyle, we must extract energy from renewable sources. The most promising renewable source, in terms of abundance, is the sun, which provides us with more than enough solar energy to fully fuel our...
FAQs
A dye-linked probe binds the selected biological target, such as an antigen, antibody, or other biomolecule. The bound probe produces an infrared signal, and researchers compare that signal with calibrated standards to estimate the target’s abundance. This connects molecular recognition with a measurable value that can be compared across samples or experimental conditions.
Infrared detection can support measurements in complex samples, where researchers may need to assess biological molecules without relying on a simple, purified preparation. The approach also permits distinct labels for multiplex measurements, allowing more than one target or biomolecular measurement to be examined within the same broader analytical framework.
Calibrated standards provide the reference needed to relate the measured infrared signal to an estimated amount of target. Without this comparison, the signal would indicate detection but would not directly support abundance estimates. Using the same calibration basis across experimental conditions helps researchers compare biomolecular levels more consistently.
A typical workflow begins by selecting a dye-linked probe for the antigen, antibody, or other biomolecule of interest. The probe is allowed to bind its target, after which the infrared signal is measured. Researchers then compare the result with calibrated standards and use the resulting estimate to evaluate differences among samples or conditions.
The assay can be directed toward antigens, antibodies, and other biomolecules, depending on the probe used. This flexibility allows the same general analytical strategy to address infection-related targets or immune-response molecules. Target selection therefore determines whether the measurement is used to characterize an immune reaction, identify an infection-associated molecule, or compare another biomolecular feature.
Researchers can use the method to characterize immune responses, identify targets associated with infection, and compare biomolecular levels across experimental conditions. For example, measurements may distinguish how target abundance varies between sample groups or study conditions. Its value in this context comes from combining target-specific binding with a sensitive infrared readout suitable for comparative analysis.