Lna Probes
Lna Probes - Related Videos
09:45
Locked Nucleic Acid Flow Cytometry-fluorescence in situ Hybridization (LNA flow-FISH): a Method for Bacterial Small RNA Detection
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Cited by 11 •
2012
A novel high-throughput method is described that enables the detection and relative quantitation of small RNA and mRNA expression from single bacterial cells using locked nucleic acid probes and flow cytometry-fluorescence in situ hybridization.
05:38
Custom-made Microdialysis Probe Design
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Cited by 9 •
2015
Microdialysis is a commonly used technique in neuroscience research. Therefore commercial probes are in great demand.In this work a probe assembly is explained in detail to build a reliable, concentric, custom-made microdialysis probe for less than $10.
13:58
Probing C84-embedded Si Substrate Using Scanning Probe Microscopy and Molecular Dynamics
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Cited by 1 •
2016
This paper reports the nanomaterial fabrication of a fullerene Si substrate inspected and verified by nanomeasurements and molecular dynamic simulation.
07:28
Measurement of Bioelectric Current with a Vibrating Probe
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Cited by 15 •
2011
The manufacture, calibration and use of non-invasive vibrating probes to measure bioelectric current in various biological systems is described.
12:16
Robust 3D DNA FISH Using Directly Labeled Probes
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Cited by 49 •
2013
We describe a robust and versatile protocol for analyzing nuclear architecture by 3D DNA FISH using directly labeled fluorescent probes.
FAQs
A methylene bridge fixes the ribose sugar in a defined conformation, reducing the structural flexibility normally available to an unmodified nucleic acid. This configuration strengthens hybridization with a complementary DNA or RNA target and increases thermal stability. The resulting probe-target interaction can remain selective even when the assay uses short target sequences.
Their increased hybridization affinity and stability can sharpen the difference between a fully complementary sequence and one containing a single-base mismatch. This discrimination is valuable when closely related microbial sequences or similar host and pathogen transcripts must be distinguished. In practice, the enhanced selectivity supports more precise recognition of sequence variants in detection assays.
LNA probes are particularly useful when the sequence of interest is short, variable, or present at low abundance. Strong binding helps maintain detectable target recognition despite limited sequence length, while selective binding can reduce ambiguity among related sequences. These properties are relevant to infection studies that examine pathogen nucleic acids or infection-associated host transcripts.
The probes can be selected for complementary pathogen or host sequences and incorporated into quantitative PCR or fluorescence in situ hybridization formats. In quantitative PCR, they support sequence-specific detection during amplification-based measurement. In fluorescence in situ hybridization, they enable visualization of complementary targets, extending analysis from sequence detection to where target nucleic acids occur.
In infection research, these probes can help identify pathogens, measure microbial transcripts, and monitor host gene-expression changes associated with infection. Their sequence selectivity is useful when pathogen targets resemble related sequences or when host responses involve transcripts that require precise discrimination. Together, these applications connect nucleic-acid detection with analysis of microbial presence and immune-related molecular responses.
Depending on the assay format, results can include quantitative measurements of microbial or host transcripts, detection of pathogen-specific sequences, or fluorescence-based evidence of target nucleic acids. Because the probes can recognize closely related sequences, the data may provide more specific interpretation than broad target detection alone, especially for short, variable, or low-abundance sequences.