Ipec-j2 Cells
Ipec-j2 Cells - Related Videos
02:53
Immunofluorescence Assay to Detect Hepatitis C Virus Replication Complexes in Infected Cells
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2026
Source: Ren, S., et al. A Protocol for Analyzing Hepatitis C Virus Replication. J. Vis. Exp. (2014).This video demonstrates an immunofluorescence-based method to detect Hepatitis C virus replication in infected hepatoma cells. The protocol involves cell fixation, antibody staining for viral protein and double-stranded RNA, and visualization using fluorophore-tagged secondary antibodies. Dual signal detection under a fluorescence microscope confirms active viral replication within the host cells.
10:11
Maturation of Human Stem Cell-derived Cardiomyocytes in Biowires Using Electrical Stimulation
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Cited by 24 •
2017
The cardiac biowire platform is an in vitro method used to mature human embryonic and induced pluripotent stem cell-derived cardiomyocytes (hPSC-CM) by combining three-dimensional cell cultivation with electrical stimulation. This manuscript presents the detailed setup of the cardiac biowire platform.
09:11
Zika Virus Infectious Cell Culture System and the In Vitro Prophylactic Effect of Interferons
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Cited by 35 •
2016
Zika Virus (ZIKV), an emerging pathogen, is linked to fetal developmental abnormalities and microcephaly. The establishment of an effective infectious cell culture system is crucial for studies of ZIKV replication as well as vaccine and drug development. In this study, various virological assays pertaining to ZIKV are illustrated and discussed.
10:04
Tractable In Vivo Reprogramming of Tumor Cells to Type 1 Conventional Dendritic Cell-like Cells
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2025
This protocol describes the in vivo reprogramming of mouse cancer cells into type 1 dendritic-like cells within the tumor microenvironment through enforced expression of the transcription factors PU.1, IRF8, and BATF3.
22:06
Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
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Cited by 7 •
2007
This protocol details the derivation of transplantable hematopoietic stem cells from mouse embryonic stem cells (ESC) and their subsequent injection into lethally irradiated recipient mice. Briefly, ESC are differentiated as embryoid bodies, which are then infected with retroviral HoxB4 and co-cultured with OP9 stromal cells and hematopoietic cytokines.
FAQs
Polarization creates distinct apical and basolateral surfaces, allowing researchers to examine intestinal events according to their side of occurrence. This organization also supports tight-junction formation and barrier measurements. As a result, experiments can distinguish effects occurring at the epithelial surface from changes associated with movement across or interaction with the underlying side of the monolayer.
Tight junctions help maintain separation between neighboring epithelial cells, while transepithelial electrical resistance provides a measurable indicator of barrier properties. Changes in this resistance can accompany altered epithelial permeability after infection or inflammatory stimulation. Together, these features let researchers evaluate whether a treatment, pathogen, or stimulus affects intestinal barrier integrity.
The two surfaces allow investigators to examine how pathogens or inflammatory stimuli interact with different sides of the epithelial barrier. This arrangement supports analysis of attachment, invasion, permeability, and microbial translocation in a spatially organized system. It therefore helps connect an initial epithelial encounter with later barrier or host-response outcomes.
Researchers culture the cells under conditions that support formation of a polarized monolayer, then assess barrier features such as tight-junction development and transepithelial electrical resistance. The established model can subsequently be exposed to pathogens or inflammatory stimuli, followed by measurements of permeability, attachment, invasion, translocation, or epithelial responses.
They are useful when the goal is to examine how an enteric pathogen interacts with intestinal epithelium in a controlled in vitro setting. The model supports separate investigation of attachment to the epithelial surface and invasion into the cell layer. These observations can clarify infection-related changes before evaluating broader barrier or host-response effects.
In immunology and infection studies, the cells provide an epithelial system for linking barrier behavior with host responses to infection or inflammatory stimuli. Researchers can examine epithelial-immune interactions alongside permeability and microbial translocation. Findings may help evaluate strategies intended to prevent or treat enteric disease while retaining experimental control and reproducibility.